Identification of putative calcium channels in skeletal muscle microsomes
Identification of putative calcium channels in skeletal muscle microsomes
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骨骼肌微粒体中推定钙通道的鉴定
DOI：
10.1016/0014-5793(82)80835-1
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发表时间：
1982
期刊：
影响因子：
3.5
通讯作者：
H. Glossmann
中科院分区：
文献类型：
--
作者：
D. Ferry;H. Glossmann
在豚鼠后肢骨骼肌匀浆中发现了标记钙拮抗剂（±）[3 H]尼莫地平的饱和结合位点。通过匀浆差速离心在微粒体沉淀物中富集结合位点。[3 H]尼莫地平结合（Kd= 1.5±0.03 nM，Bmax= 2.1 ± 0.25 pmol/蛋白，37°C）在该级分中与[3 H]哇巴因结合（6.6倍）和125 I-α-银环蛇毒素结合（5倍）共纯化（6倍）。d-顺式-地尔硫卓（而非1-顺式-地尔硫卓）通过增加Bmax刺激（±）[3 H]尼莫地平结合（ED 501 μM）。结合位点区分1，4-二氢吡啶钙拮抗剂的光学对映体和D-600的光学纯对映体。这些数据证实，通过生物化学技术，骨骼肌中存在先前通过电生理技术发现的1，4-二氢吡啶和（±）D-600可吸收钙通道。
Saturable binding sites for the labelled calcium antagonist (±)[3H]nimodipine were found in guinea‐pig hind limb skeletal muscle homogenates. Binding sites were enriched in a microsomal pellet by differential centrifugation of the homogenate. [3H]Nimodipine binding (Kd= 1.5±0.03 nM,Bmax= 2.1 ± 0.25 pmol/protein, at 37°C) copurified (6‐fold) in this fraction with [3H]ouabain binding (6.6‐fold) and125I‐α‐bungarotoxin binding (5‐fold). d‐cis‐Diltiazem (but not 1‐cis‐diltiazem) stimulated (±) [3H]nimodipine binding (ED501 μM) by increasing theBmax. Binding sites discriminated between the optical enantiomers of 1,4‐dihydropyridine calcium antagonists and the optically pure enantiomers of D‐600. The data confirm, with biochemical techniques, the presence of 1,4‐dihydropyridine and (±)D‐600 inhibitable calcium channels in skeletal muscle, previously found with electrophysiological techniques.
高亲和力外消旋放射性配体的异常平衡结合特性。
影响因子：
3.6
作者：
Burgisser,E;Hancock,AA;Lefkowitz,RJ;DeLean,A
通讯作者：
DeLean,A