Effect of repeated freeze-thaw cycles on routine plasma biochemical constituents in canine plasma
Effect of repeated freeze-thaw cycles on routine plasma biochemical constituents in canine plasma
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DOI：
10.1111/j.1939-165x.2006.tb00144.x
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发表时间：
2006-09-01
影响因子：
1.2
通讯作者：
Lefebvre, Herve Pierre
中科院分区：
文献类型：
--
作者：
Reynolds, Brice;Taillade, Benjamin;Lefebvre, Herve Pierre
背景资料：尚未在犬中评估重复冻融循环对血浆成分的影响，尽管此类程序在常规实验室实践中并不少见。目的：探讨冻融循环对健康犬血浆常规成分的影响。方法：健康成年犬6只。采集血样并置于肝素化试管中。离心后，将血浆分成5等份。将一份等分试样视为参比等分试样，并立即用于所有生化成分的测定。所有其他等分试样均储存在-20 ℃下。在1- 3天内，对3份等分试样进行1、2或3次冻融循环。最后一份等分试样在整个研究期间保持在-20 ℃下，并在第三天解冻。测定以下生化成分：葡萄糖、尿素、肌酐、总蛋白、钠、钾、氯、钙、磷酸盐、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、肌酸激酶（CK）和碱性磷酸酶（ALP）。结果：所有组分的不同等分试样之间均未观察到临床相关变化。结论：重复冻融循环不会导致犬血浆中研究的生化成分发生变化。
Background: The effect of repeated freeze-thaw cycles on plasma constituents has not been assessed in dogs, although such a procedure is not uncommon to use in routine laboratory practice. Objective: To assess the effect of freeze-thaw cycles on routine plasma constituents in healthy dogs. Methods: Six healthy adult dogs were used. Blood was sampled and placed in heparinized tubes. After centrifugation, plasma was separated into 5 aliquots. One aliquot was considered as the reference aliquot and used immediately for the assay of all of the biochemical constituents. All of the other aliquots; were stored at -20 degrees C. Three aliquots underwent 1, 2, or 3 freeze-thaw cycles during a 1- to 3-day period. The last aliquot remained at -20 degrees C throughout the study and was thawed on the third day. The following biochemical constituents were assayed: glucose, urea, creatinine, total proteins, sodium, potassium, chloride, calcium, phosphates, aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), and alkaline phosphatase (ALP). Results: No clinically relevant change was observed between the different aliquots for all of the constituents. Conclusion: Repeated freeze-thaw cycles do not cause changes in the biochemical constituents studied in canine plasma.