Ultrasensitive microchip based on smart microgel for real-time online detection of trace threat analytes
Ultrasensitive microchip based on smart microgel for real-time online detection of trace threat analytes
复制标题
基于智能微凝胶的超灵敏微芯片，用于实时在线检测痕量威胁分析物
DOI：
10.1073/pnas.1518442113
复制
发表时间：
2016-02-23
影响因子：
11.1
通讯作者：
Chu, Liang-Yin
中科院分区：
文献类型：
--
作者：
Lin, Shuo;Wang, Wei;Chu, Liang-Yin
实时检测痕量威胁分析物对于全球可持续发展至关重要。关键的挑战是如何有效地将分析物信号转换和放大为简单的读数。在这里，我们报告了一个超灵敏的微流控平台结合刺激响应智能微凝胶实时检测痕量威胁分析物。微凝胶响应于特定分析物而膨胀，从而将痕量分析物浓度转化为显著放大的流速变化信号，用于高度灵敏、快速和选择性的检测，其可以在手机上进行监测，以及时警告和终止污染。这项工作提供了一个可推广的平台，将无数的微凝胶，以实现更好的性能，实时检测各种痕量威胁分子，并可能扩大检测技术的应用范围。痕量威胁分析物的实时在线检测对于全球可持续发展至关重要，而关键挑战是如何有效地将分析物信号转换和放大为简单的读数。在这里，我们报告了一个超灵敏的微流控平台与智能微凝胶实时在线检测痕量威胁分析物。微凝胶在流动的溶液中对特定的刺激物有溶胀反应，从而有效地将刺激信号转化为显著放大的流速变化信号，从而实现高灵敏度、快速和选择性的检测。我们通过将离子可识别的微凝胶用于检测痕量Pb2+，并将我们的平台与自来水和废水的管道连接，进行实时在线Pb2+检测，以实现及时的污染预警和终止。这项工作提供了一个可推广的平台，将无数的刺激响应微凝胶，以实现更好的性能，实时在线检测各种痕量威胁分子，并可能扩大检测技术的应用范围。
Significance Real-time detection of trace threat analytes is critical for global sustainability. The key challenge is how to efficiently convert and amplify the analyte signal into simple readouts. Here we report an ultrasensitive microfluidic platform incorporated with stimuli-responsive smart microgel for real-time detection of trace threat analytes. The microgel swells in response to specific analyte, thus converting trace analyte concentration into significantly amplified signal of flow rate change for highly sensitive, fast, and selective detection, which can be monitored on cell phone for timely warning and terminating of pollution. This work provides a generalizable platform for incorporating myriad microgels to achieve ever-better performance for real-time detection of various trace threat molecules, and may expand the scope of applications of detection techniques. Real-time online detection of trace threat analytes is critical for global sustainability, whereas the key challenge is how to efficiently convert and amplify analyte signals into simple readouts. Here we report an ultrasensitive microfluidic platform incorporated with smart microgel for real-time online detection of trace threat analytes. The microgel can swell responding to specific stimulus in flowing solution, resulting in efficient conversion of the stimulus signal into significantly amplified signal of flow-rate change; thus highly sensitive, fast, and selective detection can be achieved. We demonstrate this by incorporating ion-recognizable microgel for detecting trace Pb2+, and connecting our platform with pipelines of tap water and wastewater for real-time online Pb2+ detection to achieve timely pollution warning and terminating. This work provides a generalizable platform for incorporating myriad stimuli-responsive microgels to achieve ever-better performance for real-time online detection of various trace threat molecules, and may expand the scope of applications of detection techniques.