Co-occlusion of Baculovirus Enhancin with Polyhedrin
Co-occlusion of Baculovirus Enhancin with Polyhedrin
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发表时间：
2003
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通讯作者：
Zhong Jiang
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作者：
Zhong Jiang
颗粒体病毒增强蛋白是一组能增强核型多角体病毒感染力的蛋白质。构建了表达粉纹夜蛾颗粒体病毒增强蛋白的多角体蛋白基因阴性重组体vAcBBH-TnEn，并与野生型AcMNPV联合感染SF 21细胞。在感染过程中，野生型AcMNPV的多角体可共包合到重组病毒中。SDS-PAGE结果表明，重组病毒的多角体蛋白与野生型AcMNPV的多角体蛋白结合，在感染SF 21细胞的过程中，多角体蛋白与野生型AcMNPV的多角体蛋白结合，在感染SF 21细胞的过程中，多角体蛋白与野生型AcMNPV的多角体蛋白结合，在感染SF 21细胞的过程中，多角体蛋白与野生型AcMNPV的多角体蛋白结合。免疫印迹和免疫荧光分析表明，增强蛋白和多角体蛋白同时表达，联合感染的细胞中大多数都有增强蛋白的表达和多角体的形成，对联合感染细胞中纯化的多角体的分析表明，重组增强蛋白与多角体结合。能有效提高核型多角体病毒的感染性。
Granulovirus enhancin is a group of proteins capable of increasing the infectivity of nucleopolyhedrovirus.A polyhedrin gene negative recombinant vAcBBH-TnEn expressing Trichoplusia ni granulovirus enhancin was constructed,and used to infect SF21 cells jointly with wild type AcMNPV.In the course of infection,and the polyhedra of wild type AcMNPV could be co-occluded into the recombinant virus.SDS-PAGE,immunoblot and immunofluorescence assay demonstrated that enhancin and polyhedrin were expressed simultaneously,the enhancin expression and polyhedra formation occurred in most of the jointly infected cells.Analysis of purified polyhedra from jointly infected cells showed that recombinant enhancin bound to the polyhedra.Enhancin binding to the polyhedra can effectively improve the infectivity of the nucleopolyhedrovirus.