Bio-macromolecular dynamic structures and functions, illustrated with DNA, antibody, and lipoprotein
Bio-macromolecular dynamic structures and functions, illustrated with DNA, antibody, and lipoprotein
复制标题
以DNA、抗体、脂蛋白阐释生物大分子动态结构与功能
DOI：
10.1088/1674-1056/27/2/028708
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发表时间：
2018
影响因子：
1.7
通讯作者：
Zhang Lei
中科院分区：
文献类型：
--
作者：
Gou Lu;Jin Taoli;Chen Shuyu;Li Na;Hao Dongxiao;Zhang Shengli;Zhang Lei
生物大分子，如蛋白质和核酸，是执行生命所需的基本活动的基本物质。它们的结构异质性和动态特性对于理解生物大分子的潜在功能机制至关重要。随着单分子技术和低温电子显微镜（cryo-EM）等先进技术的迅速发展，越来越多的分子结构细节和分子水平的力学特性大大提高了人们对基本生命过程的认识。本文首先对单分子法和低温电镜的基本原理进行了综述，以期对这两个领域的发展有所启发。其次，在上述两种方法的推动下，DNA、抗体和脂蛋白的动态结构和功能的探索正在进行中。最后，对生物大分子的动态结构和功能的进一步研究进行了展望，包括单分子方法和低温电镜结合分子动力学模拟。
Bio-macromolecules, such as proteins and nucleic acids, are the basic materials that perform fundamental activities required for life. Their structural heterogeneities and dynamic personalities are vital to understand the underlying functional mechanisms of bio-macromolecules. With the rapid development of advanced technologies such as single-molecule technologies and cryo-electron microscopy (cryo-EM), an increasing number of their structural details and mechanics properties at molecular level have significantly raised awareness of basic life processes. In this review, firstly the basic principles of single-molecule method and cryo-EM are summarized, to shine a light on the development in these fields. Secondly, recent progress driven by the above two methods are underway to explore the dynamic structures and functions of DNA, antibody, and lipoprotein. Finally, an outlook is provided for the further research on both the dynamic structures and functions of bio-macromolecules, through single-molecule method and cryo-EM combining with molecular dynamics simulations.