Enrichment of cell types from the rat adenohypophysis by sedimentation at unit gravity.
Enrichment of cell types from the rat adenohypophysis by sedimentation at unit gravity.
复制标题
通过单位重力沉降富集大鼠腺垂体的细胞类型。
作者：
Wesley C. Hymer;W. Evans;J. Kraicer;Andrea M. Mastro;J. Davis;E. Griswold
应用单位重力细胞分离程序，大鼠腺垂体组织。结果表明：（a）腺垂体细胞在解离和沉降后保留了其特征性的着色特性，（B）可以实现不同细胞类型的2-3倍富集，以及（c）标记的氨基酸掺入解离的腺垂体细胞与未解离的腺垂体细胞的蛋白质中的速率相似。从甲状腺切除或哺乳动物的组织中的细胞沉淀分别产生了富集的促甲状腺细胞和促乳细胞群。这种细胞分离技术为垂体细胞类型的分离、纯化和研究提供了一种新的方法。（内分泌学92：265，1973）
The application of a unit gravity cell separation procedure to rat adenohypophysial tissue is described. The results demonstrate (a) that adenohypophysial cells retain their characteristic tinctorial properties after dissociation and sedimentation, (b) that a 2–3–fold enrichment of different cell types can be achieved and (c) that the incorporation rate of labeled amino acids into the protein of dissociated versus non—dissociated adenohypophysial cells is similar. Sedimentation of cells from tissues of thyroidectomized or lactating animals yielded enriched populations of thyrotrophs and lactotrophs respectively. This cell separation technique offers a novel approach to the isolation, purification, and study of the pituitary cell types.(Endocrinology 92: 265,1973)