Dependence of FDG uptake on tumor microenvironment
Dependence of FDG uptake on tumor microenvironment
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DOI：
10.1016/j.ijrobp.2005.02.009
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发表时间：
2005-06-01
影响因子：
7
通讯作者：
Humm, JL
中科院分区：
文献类型：
--
作者：
Pugachev, A;Ruan, S;Humm, JL
目的：从微观水平探讨肿瘤摄取F-18-氟代脱氧葡萄糖的影响因素及其与肿瘤缺氧、细胞增殖、血液灌流的关系。方法与材料：荷瘤裸鼠(R3327-AT)注射F-18-脱氧葡萄糖、匹莫硝唑、溴脱氧尿苷，处死前1min注射Hoechst 33342。选定的肿瘤切片用荧光板放射自显影成像，而邻近的切片用来获得Hoechst 33342、匹莫硝唑和溴脱氧尿苷的空间分布图像。结果：对来自这些肿瘤的数据进行统计分析表明，在所研究的每个数据集中，F-18-FDG摄取与匹莫硝唑染色强度呈正相关。FDG摄取与溴脱氧尿嘧啶核苷染色强度呈负相关。此外，FDG摄取与Hoechst 33342染色强度呈负相关。结论：在所研究的Dunning前列腺癌中，FDG摄取始终与缺氧呈正相关，与细胞增殖和血流呈负相关。因此，对于所研究的肿瘤模型，较高的FDG摄取是肿瘤缺氧的指示，但既不是血流，也不是细胞增殖。©2005 Elsevier Inc.
Purpose: To investigate the factors affecting the F-18-fluorodeoxyglucose (F-18-FDG) uptake in tumors at a microscopic level, by correlating it with tumor hypoxia, cellular proliferation, and blood perfusion.Methods and Materials: Nude mice bearing Dunning prostate tumors (R3327-AT) were injected with F-18-FDG and pimonidazole, bromodeoxyuridine, and, 1 min before sacrifice, with Hoechst 33342. Selected tumor sections were imaged by, phosphor plate autoradiography, while adjacent sections were used to obtain the images of the spatial distribution of Hoechst 33342, pimonidazole, and bromodeoxyuridine. The images were co-registered and analyzed on a pixel-by-pixel basis.Results: Statistical analysis of the data obtained from these tumors demonstrated that F-18-FDG uptake was positively correlated with pimonidazole staining intensity in each data set studied. Correlation of FDG uptake with bromodeoxyuridine staining intensity was always negative. In addition, FDG uptake was always negatively correlated with the staining intensity of Hoechst 33342.Conclusions: For the Dunning prostate tumors studied, FDG uptake was always positively correlated with hypoxia and negatively correlated with both cellular proliferation and blood flow. Therefore, for the tumor model studied, higher FDG uptake is indicative of tumor hypoxia, but neither blood flow nor cellular proliferation. © 2005 Elsevier Inc.