Functional MRI of visual responses in the awake, behaving marmoset.
Functional MRI of visual responses in the awake, behaving marmoset.
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DOI：
10.1016/j.neuroimage.2015.06.090
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发表时间：
2015-10-15
期刊：
影响因子：
5.7
通讯作者：
Silva AC
中科院分区：
文献类型：
--
作者：
Hung CC;Yen CC;Ciuchta JL;Papoti D;Bock NA;Leopold DA;Silva AC
视觉脑由相互连接的皮层下和皮层结构组成，这些结构接收和处理源自视网膜的图像信息。非人类灵长类动物的视觉系统，特别是猕猴，已经被详细研究，以阐明人类感觉和感知的原理。普通绒猴（Callithrix jacchus）是一种新大陆的小型猴，作为神经科学的灵长类模型越来越受到关注。与猕猴相比，绒猴具有优势，因为它们的体积小，无脑皮层，以及越来越大的病毒和遗传操纵潜力。先前的解剖学研究和麻醉绒猴的电生理记录表明，这种物种的皮层视觉层次与包括人类在内的其他灵长类动物非常相似。然而，到目前为止，还没有人试图用功能磁共振成像系统地研究整个绒猴大脑的视觉反应。在这里，我们表明，清醒的绒猴很容易学会进行一个简单的视觉任务内孔的MRI扫描仪在功能映射实验。在30 cm水平孔中以500μm平面内分辨率在7T下进行的功能扫描显示，在整个视觉皮层和相关皮层下区域对视觉刺激产生了稳健的正血氧水平依赖性（BOLD）fMRI反应。非视觉感官领域表现出负对比视觉刺激相比，固定点只有基线。在早期视觉皮层以外的阶段，物体和面孔的结构化图像比混乱的控制图像导致更强的反应。我们的研究建立了清醒的视觉反应的功能性MRI映射，行为绒猴是简单的，有价值的高分辨率评估灵长类动物大脑的功能组织。
The visual brain is composed of interconnected subcortical and cortical structures that receive and process image information originating in the retina. The visual system of nonhuman primates, in particular macaques, has been studied in great detail in order to elucidate principles of human sensation and perception. The common marmoset (Callithrix jacchus) is a small New World monkey of growing interest as a primate model for neuroscience. Marmosets have advantages over macaques because of their small size, lissencephalic cortex, and growing potential for viral and genetic manipulations. Previous anatomical studies and electrophysiological recordings in anesthetized marmosets have shown that this species’ cortical visual hierarchy closely resembles that of other primates, including humans. Until now, however, there have been no attempts to systematically study visual responses throughout the marmoset brain using fMRI. Here we show that awake marmosets readily learn to carry out a simple visual task inside the bore of an MRI scanner during functional mapping experiments. Functional scanning at 500μm in-plane resolution in a 30 cm horizontal bore at 7T revealed robust positive blood oxygenation level-dependent (BOLD) fMRI responses to visual stimuli throughout visual cortex and associated subcortical areas. Nonvisual sensory areas showed negative contrasts to visual stimuli compared to the fixation dot only baseline. Structured images of objects and faces led to stronger responses than scrambled control images at stages beyond early visual cortex. Our study establishes functional MRI mapping of visual responses in awake, behaving marmosets is straightforward and valuable for assessing the functional organization of the primate brain at high resolution.