Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography.
Nondestructive in situ monitoring of pea seeds germination using optical coherence tomography.
复制标题
利用光学相干断层扫描技术对豌豆种子萌发的无损原位监测。
作者：
种子发芽和田间植株生长均匀是决定作物最终产量的最关键的生长阶段。豌豆（Pisum sativum L.）种子生产经常受到阻碍，由于种子休眠造成的硬种皮。这种影响主要是由于发芽差或不均匀和出苗不同步造成的。了解取水的时间过程和几个关键的发芽指标可以揭示种子萌发率和均匀性等许多特征。本文使用光学相干断层扫描（OCT），一种无创的横断面成像技术，监测整个萌发过程中的内部结构变化。在原位连续记录了豌豆（P. sativum L.）种子的一系列横截面OCT图像，以及附加的显微镜光学图像，时间超过40小时。OCT和显微镜图像分别显示了豌豆种子在萌发过程中内部结构和外部形状的变化。发现横截面OCT图像有助于识别区分豌豆种子萌发不同阶段的关键指标。因此，提出的OCT方法提供了一种快速、无损的方法来精确测量不同萌发阶段的结构指标。
Seed germination and uniform plant stand in the field are the most critical crop growth stages determining the final yield. Pea ( Pisum sativum L.) seeds production is often hampered due to the seed dormancy caused by the hard seed coat. Such effect is mainly attributed to poor or uneven germination and unsynchronised seedling emergence. Understanding the time course of water intake and several critical germination indicators can reveal many features of seed germination such as rate and uniformity. This paper used optical coherence tomography (OCT), a noninvasive and cross‐sectional imaging technique, to monitor the inner structural changes throughout the germination process. A sequence of cross‐sectional OCT images of pea ( P. sativum L.) seeds, together with additional microscopic optical images, was recorded continuously and in situ for over 40 h. OCT and microscopic images revealed the changes in the internal structure and the external shape of the pea seeds during germination, respectively. It was found that the cross‐sectional OCT images helped to identify the critical indicators distinguishing the different phases of germination pea seeds. Therefore, the presented OCT approach offers a fast and nondestructive way to precisely measure the structural indicators in different germination phases.
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DOI：
10.5958/j.0976-4038.5.1.019
发表时间：
2014-01-01
期刊：
International Journal of Bio-resource and Stress Management
影响因子：
--
作者：
Mehta, D. K.;Kanwar, H. S.;Thakur, S.
通讯作者：
Thakur, S.
影响因子：
5.5
作者：
Putaux, JL;Buléon, A;Chanzy, H
通讯作者：
Chanzy, H
影响因子：
3.8
作者：
Lin, Hungyen;Dong, Yue;Zeitler, J. Axel
通讯作者：
Zeitler, J. Axel
影响因子：
5
作者：
Joshi, Deepa;Butola, Ankit;Mehta, Dalip Singh
通讯作者：
Mehta, Dalip Singh
影响因子：
4.6
作者：
Anna, Tulsi;Chakraborty, Sandeep;Kuo, Wen-Chuan
通讯作者：
Kuo, Wen-Chuan