The effect of RGD peptides on osseointegration of hydroxyapatite biomaterials
The effect of RGD peptides on osseointegration of hydroxyapatite biomaterials
复制标题
DOI：
10.1016/j.biomaterials.2008.04.014
复制
发表时间：
2008-07-01
期刊：
影响因子：
14
通讯作者：
Bellis, Susan L.
中科院分区：
文献类型：
--
作者：
Hennessy, Kristin M.;Clem, Will C.;Bellis, Susan L.
鉴于羟基磷灰石 (HA) 生物材料在吸附促粘附蛋白方面非常有效，我们质疑用 RGD 肽功能化 HA 是否会有任何好处。在这项研究中，我们将未涂层或 RGD 涂层的 HA 盘植入大鼠胫骨中 30 分钟，以允许内源性蛋白质吸附，然后评估间充质干细胞 (MSC) 与回收的盘的相互作用。这些实验表明，当 RGD 与吸附的胫骨蛋白（包括纤连蛋白、玻连蛋白和纤维蛋白原）结合使用时，会对 MSC 粘附和存活产生明显的不利影响。此外，对植入 5 天的 HA 盘的分析表明，RGD 显着抑制总骨形成以及直接接触种植体周边的新骨数量。因此，人们普遍认为 RGD 可以促进细胞/生物材料相互作用，但它会对 HA 植入物的性能产生负面影响。总的来说，这些结果表明，对于与组织微环境高度相互作用的生物材料，RGD 的最终效果将取决于该肽的信号如何与蛋白质吸附等内源过程整合。 (c) 2008 Elsevier Ltd. 保留所有权利。
Given that hydroxyrapatite (HA) biomaterials are highly efficient at adsorbing proadhesive proteins, we questioned whether functionalizing HA with RGD peptides would have any benefit. In this study, we implanted uncoated or RGD-coated HA disks into rat tibiae for 30 min to allow endogenous protein adsorption, and then evaluated mesenchymal stem cell (MSC) interactions with the retrieved disks. These experiments revealed that RGD, when presented in combination with adsorbed tibial proteins (including fibronectin, vitronectin and fibrinogen), has a markedly detrimental effect on MSC adhesion and survival. Moreover, analyses of HA disks implanted for 5 days showed that RGD significantly inhibits total bone formation as well as the amount of new bone directly contacting the implant perimeter. Thus, RGD, which is widely believed to promote cell/biomaterial interactions, has a negative effect on HA implant performance. Collectively these results suggest that, for biomaterials that are highly interactive with the tissue microenvironment, the ultimate effects of RGD will depend upon how signaling from this peptide integrates with endogenous processes such as protein adsorption. (c) 2008 Elsevier Ltd. All rights reserved.