Brain norepinephrine and metabolites after treadmill training and wheel running in rats
Brain norepinephrine and metabolites after treadmill training and wheel running in rats
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DOI：
10.1097/00005768-199602000-00008
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发表时间：
1996-02-01
期刊：
影响因子：
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通讯作者：
Dishman, RK
中科院分区：
文献类型：
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作者：
Dunn, AL;Reigle, TG;Dishman, RK
慢性运动后脑去甲肾上腺素[NE]及其代谢物浓度的局部变化尚未被其他应激源混淆的运动方案所描述。我们检测了运动8周后额叶皮质、海马、桥-髓质和脊髓中[NE]、3-甲氧基-4-羟基苯基乙二醇[MHPG]和3,4-二羟基苯基乙二醇[DHPG]的水平。雄性Sprague-Dawley大鼠（N = 36）随机分为三组：1)24小时活动轮跑（WR）， 2) 0度倾斜跑步机跑（TR） 1小时。D（-1）在25-30米。min（-1）或3)久坐对照组(C)。浓度（nmol）用高效液相色谱法测定[NE]、[MHPG]、[DHPG]的g(-1))。计划对比（P < 0.05）表明运动训练增加琥珀酸脱氢酶活性（mmol细胞色素C降低-1）。g（-1）湿重)在TR组比目鱼肌中的含量高于WR组或C组。[NE]在TR组和WR组的桥髓质和脊髓中的含量高于C组。[DHPG]在TR组的桥髓质中含量高于C组。我们的研究结果表明，跑步机运动训练伴随着大脑去甲肾上腺素能适应，与NE细胞体和上升终端区域NE代谢增加相一致，而跑步机和轮式跑步伴随着NE细胞体和脊髓区域NE水平的增加，独立于运动训练的影响。
Regional changes in concentrations of brain norepinephrine [NE] and its metabolites after chronic exercise have not been described for exercise protocols not confounded by other stressors. We examined levels of [NE], 3-methoxy-4-hydroxyphenylglycol [MHPG], and 3,4-dihydroxyphenylglycol [DHPG] in the frontal cortex, hippocampus, pons-medulla, and spinal cord after 8 wk of exercise. Male Sprague-Dawley rats (N = 36) were randomly assigned to three conditions: 1) 24-h access to activity wheel running (WR), 2) treadmill running (TR) at 0 degrees incline for 1 h . d(-1) at 25-30 m . min(-1), or 3) a sedentary control group (C). Levels (nmol . g(-1)) of [NE], [MHPG], and [DHPG] were assayed by high performance liquid chromatography with electrochemical detection. Planned contrasts (P < 0.05) indicated that exercise training increased succinate dehydrogenase activity (mmol cytochrome C reduced-min(-1). g(-1) wet weight) in soleus muscle for TR compared with WR or C. [NE] was higher in the pons-medulla and spinal cord for both TR and WR compared with C. [DHPG] was higher in the pons-medulla for TR compared with C, and [MHPG] was higher in the frontal cortex and in the hippocampus for TR compared with C. Our results suggest that treadmill exercise training is accompanied by brain noradrenergic adaptations consistent with increased metabolism of NE in areas containing NE cell bodies and ascending terminals, whereas treadmill running and wheel running are accompanied by increases in levels of NE in the areas of NE cell bodies and the spinal cord, independently of an exercise training effect.