Mesenchymal Stem Cell-Based Therapy Improves Lower Limb Movement After Spinal Cord Ischemia in Rats.
Mesenchymal Stem Cell-Based Therapy Improves Lower Limb Movement After Spinal Cord Ischemia in Rats.
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DOI：
10.1016/j.athoracsur.2017.12.014
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发表时间：
2018-05
期刊：
影响因子：
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通讯作者：
Shinya Takahashi;K. Nakagawa;Mayumi Tomiyasu;A. Nakashima;Keijiro Katayama;Takeshi Imura;Bagus Herlambang-Bag
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文献类型：
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作者：
Shinya Takahashi;K. Nakagawa;Mayumi Tomiyasu;A. Nakashima;Keijiro Katayama;Takeshi Imura;Bagus Herlambang-Bag
脊髓缺血是胸主动脉和胸腹主动脉手术后的严重并发症。在这项研究中，我们的目的是探讨骨髓间充质干细胞（MSC），这具有再生能力，并发挥旁分泌作用的受损组织，注入大鼠模型的脊髓缺血-再灌注injury. Methods 45 Sprague-Dawley大鼠分为假手术，磷酸盐缓冲盐水（PBS），MSC组。后两组通过球囊阻断胸主动脉诱导脊髓缺血。然后立即将MSC和PBS分别注射到MSC和PBS组的左颈动脉中。在6和24小时评价后肢运动功能。脊髓缺血再灌注损伤后24小时被删除，组织学和免疫组化分析和实时聚合酶链反应assessments.ResultsRats在MSC和PBS组显示松弛性截瘫/截瘫术后。在缺血再灌注损伤后6小时和24小时，MSC组的后肢功能明显好于PBS组（p< 0.05）。PBS组脊髓末端脱氧核苷酸转移酶dUTP缺口末端标记阳性神经元细胞数及Bcl-2/Bcl-2比值均显著高于MSC组（p< 0.05）。结论脊髓缺血再灌注损伤后即刻经动脉注射MSC治疗，可通过抑制脊髓神经细胞凋亡，改善下肢功能。
BackgroundSpinal cord ischemia is a devastating complication after thoracic and thoracoabdominal aortic operations. In this study, we aimed to investigate the effects of mesenchymal stem cells (MSCs), which have regenerative capability and exert paracrine actions on damaged tissues, injected into rat models of spinal cord ischemia–reperfusion injury.MethodsForty-five Sprague-Dawley rats were divided into sham, phosphate-buffered saline (PBS), and MSC groups. Spinal cord ischemia was induced in the latter two groups by balloon occlusion of the thoracic aorta. MSCs and PBS were then immediately injected into the left carotid artery of the MSC and PBS groups, respectively. Hindlimb motor function was evaluated at 6 and 24 hours. The spinal cord was removed at 24 hours after ischemia–reperfusion injury, and histologic and immunohistochemical analyses and real-time polymerase chain reaction assessments were performed.ResultsRats in the MSC and PBS groups showed flaccid paraparesis/paraplegia postoperatively. Hindlimb function was significantly better at 6 and 24 hours after ischemia–reperfusion injury in the MSC group than in the PBS group (p< 0.05). The number of terminal deoxynucleotidyl transferase dUTP nick end labeling-positive neuron cells in the spinal cord and the ratio ofBaxtoBcl2were significantly larger (p< 0.05) in the PBS group than in the MSC group. The injected MSCs were observed in the spinal cord 24 hours after ischemia–reperfusion injury.ConclusionsThe MSC therapy by transarterial injection immediately after spinal cord ischemia–reperfusion injury may improve lower limb function by preventing apoptosis of neuron cells in the spinal cord.