Matrin-3 plays an important role in cell cycle and apoptosis for survival in malignant melanoma
Matrin-3 plays an important role in cell cycle and apoptosis for survival in malignant melanoma
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DOI：
10.1016/j.jdermsci.2020.08.013
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发表时间：
2020-11-01
影响因子：
4.6
通讯作者：
Kita, Kanako
中科院分区：
文献类型：
--
作者：
Kuriyama, Haruka;Fukushima, Satoshi;Kita, Kanako
背景资料：先前的研究表明，matrin-3是维持成纤维细胞生长因子2（FGF 2）介导的神经干细胞（NSC）未分化的重要组成部分，使用蛋白质组学方法。恶性黑色素瘤（MM）是由发育过程中神经嵴干细胞产生的黑素细胞引起的。此外，据报道，FGF 2的表达与MM的进展呈正相关。目的：我们预计，matrin-3，作为一个下游成分的FGF 2，可能与MM的侵袭性或分化。方法：Matrin-3的表达测定在人黑色素瘤患者组织和人MM细胞系。我们分析了matrin-3 siRNA对人MM细胞系增殖的影响，并重点关注细胞周期进程和凋亡。结果：Matrin-3在MM中高表达，Matrin-3基因敲低可抑制MM细胞的增殖。此外，我们发现，matrin-3敲低导致细胞在G1期的积累和凋亡细胞number.Conclusion的增加：我们的研究结果表明，matrin-3可能是一个新的治疗MM的治疗靶点。（C）2020日本社会调查皮肤病。Elsevier B. V.出版，保留所有权利。
Background: A previous study revealed that matrin-3 is an essential component in maintaining fibroblast growth factor 2 (FGF2)-mediated undifferentiation of neural stem cells (NSCs) using a proteomics approach. Malignant melanoma (MM) arises from melanocytes that originate from neural crest stem cells during development. Additionally, it has been reported that the expression of FGF2 is positively correlated with the progression of MM.Objective: We expected that matrin-3, as a downstream component of FGF2, might be associated with the aggressiveness or differentiation of MM.Methods: Matrin-3 expression was measured in human melanoma patient tissues and human MM cell lines. We analyzed the effect of matrin-3 siRNA on the proliferation of human MM cell lines and focused on cell cycle progression and apoptosis. We carried out in vivo xenograft tumor experiments by implanting A375 cells transfected with matrin-3 shRNA.Results: Matrin-3 was highly expressed in MM, and matrin-3 knockdown inhibited the proliferation of melanoma cellsin vivo and in vitro. Furthermore, we found that matrin-3 knockdown led to an accumulation of cells in the G1 phase and an increase in apoptotic cell number.Conclusion: Our results suggest that matrin-3 could be a new therapeutic target for the treatment of MM. (C) 2020 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.