Bioengineering human skeletal muscle models: Recent advances, current challenges and future perspectives.
Bioengineering human skeletal muscle models: Recent advances, current challenges and future perspectives.
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DOI：
10.1016/j.yexcr.2022.113133
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发表时间：
2022-04
影响因子：
3.7
通讯作者：
Y. Jiang;T. Torun;S. Maffioletti;Andrea Serio;Francesco Saverio Tedesco
中科院分区：
文献类型：
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作者：
Y. Jiang;T. Torun;S. Maffioletti;Andrea Serio;Francesco Saverio Tedesco
人类骨骼肌组织的工程模型为研究和开发急性肌肉损伤的治疗策略提供了独特的转化机会，并为严重神经肌肉和肌肉骨骼疾病的体外研究建立了个性化和精确的医学平台。可以分离、产生、扩增几种肌源性和非肌源性细胞类型，并将其与支架和生物材料组合以实现该目的。新的生物制造策略，其中包括外源性刺激，以提高组织的成熟，承诺实现不断增加的组织功能化程度无论是在体内和体外。在这里，我们回顾了最近的进展，目前的挑战和未来的前景，建立人类骨骼肌组织“在一个盘子里”，重点是细胞成分和体外疾病建模的应用。我们还简要讨论了3D生物打印、器官芯片和类器官等新兴技术在未来研究中可能必须规避当前技术障碍的影响。
Engineering models of human skeletal muscle tissue provides unique translational opportunities to investigate and develop therapeutic strategies for acute muscle injuries, and to establish personalised and precision medicine platforms forin vitrostudies of severe neuromuscular and musculoskeletal disorders. Several myogenic and non-myogenic cell types can be isolated, generated, amplified and combined with scaffolds and biomaterials to achieve this aim. Novel bio-fabrication strategies, which include exogenous stimuli to enhance tissue maturation, promise to achieve an ever-increasing degree of tissue functionalisation bothin vivoandin vitro. Here we review recent advances, current challenges and future perspectives to build human skeletal muscle tissue “in a dish”, focusing on the cellular constituents and on applications forin vitrodisease modelling. We also briefly discuss the impact that emerging technologies such as 3D bioprinting, organ-on-chip and organoids might have to circumvent current technical hurdles in future studies.