ABCG2/BCRP gene expression is related to epithelial-mesenchymal transition inducer genes in a papillary thyroid carcinoma cell line (TPC-1)
ABCG2/BCRP gene expression is related to epithelial-mesenchymal transition inducer genes in a papillary thyroid carcinoma cell line (TPC-1)
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DOI：
10.1530/jme-14-0051
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发表时间：
2014-06-01
影响因子：
3.5
通讯作者：
de Leiva, A.
中科院分区：
文献类型：
--
作者：
Mato, E.;Gonzalez, C.;de Leiva, A.
肿瘤恶性与上皮间质转化（EMT）过程和化疗耐药相关。然而，人们对甲状腺肿瘤进展中EMT与多药耐药基因之间的关系知之甚少。我们研究了 ABCG2/BCRP 基因的表达是否与 ZEB1 和其他参与肿瘤去分化的 EMT 诱导基因相关。我们建立了一个表达来自甲状腺乳头状癌细胞系 (TPC-1) 的 ABCG2/BCRP 基因的细胞亚群，即所谓的 TPC-1 MITO 耐药亚系。这些 TPC-1 选择的细胞中最相关的发现是 ZEB1 和 TWIST1 统计上显着的上调（分别为 35 倍和 15 倍），波形蛋白和 SNAIL1 的相对表达没有变化，并且 E-钙粘蛋白没有表达。 TPC-1 MITO 抗性亚系比亲代细胞表现出更快的迁移和更强的侵袭能力，这与生存素 (BIRC5) 基因的显着上调相关（双重变化，P
Tumor malignancy is associated with the epithelial-mesenchymal transition (EMT) process and resistance to chemotherapy. However, little is known about the relationship between the EMT and the multidrug-resistance gene in thyroid tumor progression. We investigated whether the expression of the ABCG2/BCRP gene is associated with ZEB1 and other EMT inducer genes involved in tumor dedifferentiation. We established a subpopulation of cells that express the ABCG2/BCRP gene derived from the thyroid papillary carcinoma cell line (TPC-1), the so-called TPC-1 MITO-resistant subline. The most relevant findings in these TPC-1 selected cells were a statistically significant upregulation of ZEB1 and TWIST1 (35- and 15-fold change respectively), no changes in the relative expression of vimentin and SNAIL1, and no expression of E-cadherin. The TPC-1 MITO-resistant subline displayed a faster migration and greater invasive ability than parental cells in correlation with a significant upregulation of the survivin (BIRC5) gene (twofold change, P