The transepithelial voltage of the isolated anterior stomach of mosquito larvae (Aedes aegypti):: pharmacological characterization of the serotonin-stimulated cells
The transepithelial voltage of the isolated anterior stomach of mosquito larvae (Aedes aegypti):: pharmacological characterization of the serotonin-stimulated cells
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DOI：
10.1242/jeb.00964
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发表时间：
2004-05-01
影响因子：
2.8
通讯作者：
Moffett, DF
中科院分区：
文献类型：
--
作者：
Onken, H;Moffett, SB;Moffett, DF
用“半开放”的制备方法研究了分离和灌注的蚊子幼虫（埃及伊蚊）前胃的管腔阴性经上皮电压（V-te），其中将肠道的一端连接到灌注移液管上，另一端保持对浴液的开放。所有实验均采用血清素刺激制剂进行。加入2.5 mmol l（-1）二硝基苯酚后，V-te被消除，并依赖于Cl-的存在。Na+取代实验表明，V-te的大部分依赖于这种阳离子在上皮血淋巴侧的存在。在培养液中加入10 mmol l（-1）康那霉素（78+/-6%的抑制作用）或2.5 mmol l（-1）瓦阿因（15+/-2%的抑制作用）部分抑制Vte。DPC （0.5 mmol l(-1)）或DIDS （0.1 mmol l(-1)）作用于上皮的血淋巴侧时，降低了V-te（分别为对照组的49+/-8%或78+/-3%）。当存在于上皮两侧时，这些抑制剂导致Vie进一步降低（分别为对照组的23+/-4%或35+/-4%）。血淋巴侧呋塞米（0.1 mmol l(-1)）或BaCl2 （5 mmol l(-1)）分别使Vte降低13+/-3%或23+/-4%。当应用于上皮的血淋巴侧时，阿米洛利（0.2 mmol l(-1)）显着降低了对照的35+/-6%的Vte，而当随后也应用于上皮的管腔侧时，该药物没有进一步的影响。上述结果为蚊幼虫前胃碱化过程中NaHCO3分泌/HCl吸收细胞机制的扩展模型奠定了基础。
The lumen-negative transepithelial voltage (V-te) of the isolated and perfused anterior stomach of mosquito larvae (Aedes aegypti) was studied with a 'semi-open' preparation in which one end of the gut was ligated onto a perfusion pipette and the other end remained open to the bath. All experiments were performed with serotonin-stimulated preparations. V-te was abolished after addition of 2.5 mmol l(-1) dinitrophenol and depended on the presence of Cl-. Na+ substitution experiments showed that a major part of V-te depended on the presence of this cation in the hemolymph side of the epithelium. Addition of 10 mumol l(-1) concanamycin (78+/-6% inhibition) or 2.5 mmol l(-1) ouabain (15+/-2% inhibition) to the bath partially inhibited Vte. DPC (0.5 mmol l(-1)) or DIDS (0.1 mmol l(-1)) reduced V-te when applied to the hemolymph side of the epithelium (to 49+/-8% or 78+/-3% of the control, respectively). When present on both sides of the epithelium, these inhibitors caused further Vie reductions (to 23+/-4% or 35+/-4% of the control, respectively). Hemolymph-side furosemide (0.1 mmol l(-1)) or BaCl2 (5 mmol l(-1)) reduced Vte by 13+/-3% or 23+/-4% of the control, respectively. When applied to the hemolymph side of the epithelium, amiloride (0.2 mmol l(-1)) significantly decreased Vte by 35+/-6% of the control, whereas the drug caused no further effect when it was subsequently also applied to the luminal side of the epithelium. The above results are the basis for an extended model for the cellular mechanisms of NaHCO3 secretion/HCl absorption involved in alkalization of the anterior stomach of mosquito larvae.