Sphingomonas metalli sp nov., isolated from an abandoned lead-zinc mine
Sphingomonas metalli sp nov., isolated from an abandoned lead-zinc mine
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金属鞘氨醇单胞菌 nov.，从废弃的铅锌矿中分离出来
DOI：
10.1099/ijsem.0.000989
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发表时间：
2016
影响因子：
2.8
通讯作者：
Zhu Hong-Hui
中科院分区：
文献类型：
--
作者：
Feng Guang-Da;Yang Song-Zhen;Xiong Xiong;Li Hua-Ping;Zhu Hong-Hui
从广东省梅州市某废弃铅锌矿中分离到一株革兰氏阴性非运动细菌9 O-5 T。该分离株呈橙色，需氧，氧化酶和过氧化氢酶阳性。系统发育分析表明，菌株9 O-5 T属于鞘氨醇单胞菌属（SphingomonasabaciDSM 15867 T），与鞘氨醇单胞菌（SphingomonasphyllosphaeraeFA 2 T）和广东兴单胞菌（Shingomonasguangdongensis）9 NM-8 T的亲缘关系最近，同源性分别为97.6%、96.9%和96.8%。菌株9 O-5 T与S之间的平均DNA-DNA相关性。DSM 15867 T的abaci仅为47.1 ± 4.9%。主要脂肪酸为C18：1ω 7 c、C14：02-OH和总特征3（C16：1ω 7 c和/或C16：1ω6c）。它含有Q-10作为主要的呼吸醌和sym-homospermidine作为主要的多胺。极性脂质为鞘糖脂、磷脂酰乙醇胺、磷脂酰甘油、二磷脂酰甘油、5种未鉴别磷脂和6种未鉴别脂质。菌株9 O-5 T基因组DNA G+C含量为69.1 ± 0.1mol%。 根据多相分类学研究结果，9 O-5 T菌株应被认为是鞘氨醇单胞菌属的一个新种，其名称为金属鞘氨醇单胞菌。十一是建议。模式菌株为9 O-5 T（= CGMCC 1.15330T= KCTC 42759 T）。
A Gram-stain-negative and non-motile bacterial strain designated 9O-5Twas isolated from an abandoned lead–zinc mine in Meizhou, Guangdong Province, southern China. The isolate was orange-pigmented, aerobic, and oxidase- and catalase-positive. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain 9O-5Tbelongs to the genusSphingomonasand was closely related toSphingomonas abaciDSM 15867T(97.6 % similarity),Sphingomonas phyllosphaeraeFA2T(96.9 %) andShingomonas guangdongensis9NM-8T(96.8 %). Mean DNA–DNA relatedness between strain 9O-5TandS. abaciDSM 15867Twas only 47.1 ± 4.9 %. The major fatty acids were C18 : 1ω7c, C14 : 02-OH and summed feature 3 (C16 : 1ω7cand/or C16 : 1ω6c). It contained Q-10 as the predominant respiratory quinone andsym-homospermidine as the major polyamine. The polar lipids were sphingoglycolipid, phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, five unidentified phospholipids and six unidentified lipids. The genomic DNA G+C content of strain 9O-5Twas 69.1 ± 0.1 mol%. Based on the data from this polyphasic taxonomic study, strain 9O-5Tshould be considered as representing a novel species of the genusSphingomonas, for which the nameSphingomonas metallisp. nov. is proposed. The type strain is 9O-5T( = CGMCC 1.15330T= KCTC 42759T).