Expression of B7-H1 and B7-DC on the airway epithelium is enhanced by double-stranded RNA
Expression of B7-H1 and B7-DC on the airway epithelium is enhanced by double-stranded RNA
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DOI：
10.1016/j.bbrc.2005.02.161
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发表时间：
2005-04-29
影响因子：
3.1
通讯作者：
Nakanishi, Y
中科院分区：
文献类型：
--
作者：
Tsuda, M;Matsumoto, K;Nakanishi, Y
呼吸道中的病毒感染引起各种免疫应答，包括Th 1和Th 2应答，其部分由双链RNA（dsRNA）（其复制的病毒产物）启动。B7-H1（PD-L1）和B7-DC（PD-L2）是B7家族分子，与淋巴细胞上的程序性死亡-1（PD-1）结合，并参与外周耐受。我们研究了dsRNA对气道上皮细胞系上B7-H1和B7-DC表达的影响。B7-H1和B7-DC在细胞上组成性表达，并且它们的表达通过用病毒dsRNA的类似物，聚肌胞苷酸刺激而显著上调。B7-H1和B7-DC也通过用IFN-γ、IL-13和来自T细胞克隆的上清液刺激而上调。需要相对高浓度的地塞米松（1 μ M）来抑制B7-H1或B7-DC的上调。这些结果表明，上皮B7-H1和B7-DC在气道中的病毒相关免疫应答中发挥作用。(c)2005年爱思唯尔公司All rights reserved.
Viral infection in the airway provokes various immune responses, including Th1 and Th2 responses, which are partly initiated by double-stranded RNA (dsRNA), a viral product for its replication. B7-H1 (PD-L1) and B7-DC (PD-L2) are B7-family molecules that bind to programmed death-1 (PD-1) on lymphocytes and are implicated in peripheral tolerance. We investigated the effect of dsRNA on the expression of B7-H1 and B7-DC on airway epithelial cell lines. B7-H1 and B7-DC were constitutively expressed on the cells, and their expression was profoundly upregulated by stimulation with an analog of viral dsRNA, polyinosinic-polycytidylic acid. B7-H1 and B7-DC were also upregulated by stimulation with IFN-gamma, IL-13, and the supernatant from T cell clones. A relatively high concentration of dexamethasone (1 mu M) was required to suppress the upregulation of B7-H1 or B7-DC. These results suggest that epithelial B7-H1 and B7-DC play a role in virus-associated immune responses in the airways. (c) 2005 Elsevier Inc. All rights reserved.