Ectopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and Arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traits.
Ectopic phytocystatin expression leads to enhanced drought stress tolerance in soybean (Glycine max) and Arabidopsis thaliana through effects on strigolactone pathways and can also result in improved seed traits.
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DOI：
10.1111/pbi.12193
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发表时间：
2014-09
影响因子：
13.8
通讯作者：
M. Quain;M. E. Makgopa;Belen Marquez-Garcia;G. Comadira;N. Fernandez‐Garcia;E. Olmos;D. Schnaubelt;K. Kunert;C. Foyer
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文献类型：
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作者：
M. Quain;M. E. Makgopa;Belen Marquez-Garcia;G. Comadira;N. Fernandez‐Garcia;E. Olmos;D. Schnaubelt;K. Kunert;C. Foyer
异位半胱氨酸抑制素表达长期以来一直用于植物病虫害管理，但这些抑制剂的靶点半胱氨酸蛋白酶也可能在控制植物寿命和胁迫耐受性方面具有重要功能，但尚未得到充分的研究。因此，我们表征了水稻胱抑素oryacystatin - i （OCI）表达对作物（大豆）和模式（拟南芥）植物生长发育和耐受性的影响。大豆异位OCI的表达促进了营养发育后期茎部分支和叶片叶绿素积累，提高了种子蛋白质含量，降低了独角麦内酯合成酶编码mrna的丰度。表达oci的拟南花表现出生长缓慢的表型，开花时叶片数量增加，枝条分枝增强。oci依赖性的半胱氨酸蛋白酶抑制增强了大豆和拟蓝的抗旱性，在表达oci的大豆品系中，光合CO2同化对干旱诱导抑制的敏感性要低得多。异位表达OCI或用半胱氨酸蛋白酶抑制剂E64处理可增加拟南草侧根密度。E64处理也增加了独行内酯信号缺陷的max2-1突变体的侧根密度，但没有增加独行内酯合成缺陷的max3-9突变体的侧根密度。综上所述，这些数据提供了oci抑制的半胱氨酸蛋白酶通过影响独角酯内酯参与控制生长和胁迫耐受性的证据。由此可见，半胱氨酸蛋白酶是调控植物生长发育和逆境耐受性以及种子品质性状的重要靶点。
Ectopic cystatin expression has long been used in plant pest management, but the cysteine protease, targets of these inhibitors, might also have important functions in the control of plant lifespan and stress tolerance that remain poorly characterized. We therefore characterized the effects of expression of the rice cystatin, oryzacystatin-I (OCI), on the growth, development and stress tolerance of crop (soybean) and model (Arabidopsis thaliana) plants. Ectopic OCI expression in soybean enhanced shoot branching and leaf chlorophyll accumulation at later stages of vegetative development and enhanced seed protein contents and decreased the abundance of mRNAs encoding strigolactone synthesis enzymes. The OCI-expressing A. thaliana showed a slow-growth phenotype, with increased leaf numbers and enhanced shoot branching at flowering. The OCI-dependent inhibition of cysteine proteases enhanced drought tolerance in soybean and A. thaliana, photosynthetic CO2 assimilation being much less sensitive to drought-induced inhibition in the OCI-expressing soybean lines. Ectopic OCI expression or treatment with the cysteine protease inhibitor E64 increased lateral root densities in A. thaliana. E64 treatment also increased lateral root densities in the max2-1 mutants that are defective in strigolactone signalling, but not in the max3-9 mutants that are defective in strigolactone synthesis. Taken together, these data provide evidence that OCI-inhibited cysteine proteases participate in the control of growth and stress tolerance through effects on strigolactones. We conclude that cysteine proteases are important targets for manipulation of plant growth, development and stress tolerance, and also seed quality traits.