Genome-scale loss-of-function screening with a lentiviral RNAi library
Genome-scale loss-of-function screening with a lentiviral RNAi library
复制标题
DOI：
10.1038/nmeth924
复制
发表时间：
2006-09-01
期刊：
影响因子：
48
通讯作者：
Sabatini, David M.
中科院分区：
文献类型：
--
作者：
Root, David E.;Hacohen, Nir;Sabatini, David M.
RNA干扰（RNAi）在哺乳动物细胞中具有功能这一发现促使我们成立了RNA干扰联盟（TRC），其目标是通过开发基因组规模的RNAi文库以及使用这些文库的方法，实现大规模的功能缺失筛选。这些资源构成了博德研究所创建的功能缺失筛选平台的基础。我们的人类和小鼠文库目前包含超过135,000个慢病毒克隆，靶向27,000个基因。初步的筛选工作已经证明，这些文库和方法是用于高通量慢病毒RNAi筛选的实用且强大的工具。
The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain > 135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.