UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength
UV-LED irradiation reduces the infectivity of herpes simplex virus type 1 by targeting different viral components depending on the peak wavelength
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DOI：
10.1016/j.jphotobiol.2022.112410
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发表时间：
2022-02-19
影响因子：
5.4
通讯作者：
Takahashi, Akira
中科院分区：
文献类型：
--
作者：
Bui, Thi Kim Ngan;Mawatari, Kazuaki;Takahashi, Akira
1 型单纯疱疹病毒 (HSV-1) 是一种主要感染人类的包膜病毒。鉴于其全球患病率较高，消毒对于降低感染风险至关重要。紫外线发光二极管（UV-LED）是具有不同峰值波长的环保照射模块，但UV-LED照射所降解的分子尚未明确。为了识别 UV-LED 的目标病毒分子，我们将 HSV-1 悬浮液暴露于波长 260、280、310 和 365 nm 的 UV-LED 照射下，并测量宿主细胞中的病毒 DNA、蛋白质和脂质损伤和感染性。所有 UV-LED 均通过抑制宿主细胞转录而显着减少病毒感染，但 260 和 280 nm UV-LED 的杀病毒效率明显强于 310 和 365 nm UV-LED。同时，260 和 280 nm UV-LED 诱导病毒 DNA 光产物的形成以及病毒蛋白和某些磷酸甘油脂的降解。与 260 和 280 nm UV-LED 不同，310 和 365 nm UV-LED 降低了病毒蛋白水平，但它们没有显着改变病毒 DNA 光产物和亲脂性代谢物的水平。这些结果表明，UV-LED 根据峰值波长靶向不同的病毒分子，从而降低 HSV-1 的感染性。这些发现有助于优化 UV-LED 照射以实现病毒灭活。
Herpes simplex virus type 1 (HSV-1) is an enveloped virus that mainly infects humans. Given its high global prevalence, disinfection is critical for reducing the risk of infection. Ultraviolet-light-emitting diodes (UV-LEDs) are eco-friendly irradiating modules with different peak wavelengths, but the molecules degraded by UV-LED irradiation have not been clarified. To identify the target viral molecules of UV-LEDs, we exposed HSV-1 sus-pensions to UV-LED irradiation at wavelengths of 260-, 280-, 310-, and 365-nm and measured viral DNA, pro-tein, and lipid damage and infectivity in host cells. All UV-LEDs substantially reduced by inhibiting host cell transcription, but 260-and 280-nm UV-LEDs had significantly stronger virucidal efficiency than 310-and 365-nm UV-LEDs. Meanwhile, 260-and 280-nm UV-LEDs induced the formation of viral DNA photoproducts and the degradation of viral proteins and some phosphoglycerolipid species. Unlike 260-and 280-nm UV-LEDs, 310-and 365-nm UV-LEDs decreased the viral protein levels, but they did not drastically change the levels of viral DNA photoproducts and lipophilic metabolites. These results suggest that UV-LEDs reduce the infectivity of HSV-1 by targeting different viral molecules based on the peak wavelength. These findings could facilitate the optimization of UV-LED irradiation for viral inactivation.