NUCLEOTIDE-SEQUENCES OF NORMAL AND REARRANGED RNA SEGMENTS-10 OF HUMAN ROTAVIRUSES
NUCLEOTIDE-SEQUENCES OF NORMAL AND REARRANGED RNA SEGMENTS-10 OF HUMAN ROTAVIRUSES
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DOI：
10.1099/0022-1317-73-3-633
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发表时间：
1992-03-01
影响因子：
3.8
通讯作者：
DESSELBERGER, U
中科院分区：
文献类型：
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作者：
BALLARD, A;MCCRAE, MA;DESSELBERGER, U
从慢性感染的免疫缺陷儿童分离的A组轮状病毒的正常和重排的RNA片段10通过聚合酶链反应扩增为全长cDNA拷贝，并随后进行克隆和测序。与正常RNA片段10的核苷酸序列相比，重排形式在其3'端含有部分非编码重复和几个点突变。正常的RNA片段10与牛轮状病毒相似。
Normal and rearranged RNA segments 10 of group A rotaviruses isolated from a chronically infected immunodeficient child were amplified by the polymerase chain reaction as full-length cDNA copies, and were subsequently cloned and sequenced. Compared with the nucleotide sequence of the normal RNA segment 10, the rearranged form contains a partial non-coding duplication at its 3' end and several point mutations. The normal RNA segment 10 was similar to that of bovine rotavirus.