Sequential patterns of chemokine- and chemokine receptor-synthesis following vessel wall injury in porcine coronary arteries
Sequential patterns of chemokine- and chemokine receptor-synthesis following vessel wall injury in porcine coronary arteries
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DOI：
10.1016/j.atherosclerosis.2006.05.050
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发表时间：
2007-05-01
期刊：
影响因子：
5.3
通讯作者：
Wilcox, Josiah N.
中科院分区：
文献类型：
--
作者：
Jabs, Alexander;Okamoto, Ei-ichi;Wilcox, Josiah N.
炎症在血管修复中起着核心作用，并在血管成形术后扩散到血管周围组织（PVT）。趋化因子（CK）和趋化因子受体（CKR）是炎症趋化的关键决定因素。我们试图评估ckccl2和CXCL2，以及CKR CCR2、CCR5和CXCR4在球囊损伤的猪冠状动脉中的动脉和血管周围表达。采用原位杂交（ISH）技术检测血管成形术后血管细胞中表达特异性CK和CKR mRNA的情况，并采用实时RT-PCR技术定量检测血管成形术后PVT中CCL2的表达情况。损伤后2 ~ 24小时，CCL2在PVT中达到峰值，与局部巨噬细胞活化一致。24 ~ 3 d时中膜和外膜表达上调，7 d时新生内膜表达上调。CXCL2在2和4 h的培养基中检测到，也在一些新生内膜细胞中检测到。CCR2和CCR5在PVT中分别在24 h和3 d达到最大值。第2天和第3天表达转移到中膜和外膜，第7天表达转移到新生内膜和外膜，第14天表达水平较低。CXCR4在PVT中表达水平较低，但在第2天和第3天时中膜和外膜表达上调，在第7天时新内膜和外膜表达上调。总之，PVT是血管成形术后早期炎性CK和CKR的主要来源。研究发现，在冠状动脉损伤反应过程中，CK-和ckr合成的特定序列模式可能通过时空差异表达来调节相特异性趋化性。2006爱思唯尔爱尔兰有限公司版权所有。
Inflammation plays a central role in vascular repair, and spreads into perivascular tissue (PVT) following angioplasty. Chemokines (CK) and chemokine receptors (CKR) are key determinants of inflammatory chemotaxis. We sought to assess the arterial and perivascular expression of the CK CCL2 and CXCL2, and the CKR CCR2, CCR5, and CXCR4 in balloon-injured porcine coronary arteries. Vascular cells that express specific CK and CKR mRNA during post-angioplasty time course were detected by in situ hybridization (ISH), and expression was quantified by real time RT-PCR in PVT. CCL2 was maximal in PVT from 2 to 24 It post injury, coincident with local macrophage-activation. Expression was upregulated in media and adventitia from 24 It to 3 days, and in neointima at 7 days. CXCL2 was detected in media at 2 and 4 h, and also in some neointimal cells. CCR2 and CCR5 were maximal in PVT at 24 h and 3 days, respectively. Expression shifted to media and adventitia at 2 and 3 days, and to neointima and adventitia at 7 days, and was low at 14 days. CXCR4 was low in PVT, but was upregulated in media and adventitia at 2 and 3 days, as well as in neointima and adventitia at 7 days.In conclusion, PVT is the primary source of inflammatory CK and CKR early post-angioplasty. Specific sequential patterns of CK- and CKR-synthesis are identified that may regulate phase-specific chemotaxis by spatio-temporally differential expression during coronary response to injury. (c) 2006 Elsevier Ireland Ltd. All rights reserved.