Application of Fluorescent Staining of Chromosomes to Genetic Studies in Citrus
Application of Fluorescent Staining of Chromosomes to Genetic Studies in Citrus
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发表时间：
2007
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通讯作者：
Masashi Yamamoto
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作者：
Masashi Yamamoto
本文综述了鸟嘌呤 - 胞嘧啶特异性荧光染料色霉素A3（CMA）对柑橘染色体染色在遗传学研究中的应用。当进行CMA染色时，证明了柑橘染色体中存在具有高度多样性和杂合性的特征性CMA带型模式。在相关物种中也观察到了类似的CMA带型模式。因此，染色体的CMA带型模式被认为为柑橘的系统发育研究提供了非常重要的信息。通过CMA显带方法，柑橘的染色体鉴定也取得了快速进展。以荧光带的相对大小为指标，对呈现相同CMA带型模式的染色体进行了分离和分类。通过这种方法鉴定了单倍体柑橘的所有九条染色体。对亲本材料及其后代的染色体构型分析对于柑橘的遗传改良是有效的。由于特征性染色体是从亲本传递下来的，因此它们可以用作亲子关系的标记。特别是，CMA染色体染色是倍性操作的一种非常强大的工具。使用CMA染色的优点是可以一次性清晰地观察到整个染色体构型。
The application of staining with the guanine-cytosine specific fluorochrome chromomycin A3 (CMA) of citrus chromosomes to genetic studies is reviewed. When CMA staining is performed, the existence of characteristic CMA banding patterns with high levels of diversity and heterozygosity in citrus chromosomes is demonstrated. Similar CMA banding patterns have been observed in related species. Thus, CMA banding patterns of chromosomes have been considered to provide very important information for phylogenic studies in citrus. Chromosomal identification of citrus has also progressed rapidly by means of the CMA banding method. Chromosomes exhibiting the same CMA banding pattern were separated and classified using the relative sizes of fluorescent bands as indices. All nine chromosomes of haploid citrus were identified by this method. Analysis of the chromosomal configuration of parental materials and their progeny was efficient for genetic improvement in citrus. Characteristic chromosomes could be used as a marker for the parent-progeny relationship since these chromosomes are transmitted from the parents. In particular, CMA chromosome staining is a very powerful tool for ploidy manipulation. The advantage of using CMA staining is that the entire chromosomal configuration can be clearly observed at once.