Suppression of the homeobox gene HDTF1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton
Suppression of the homeobox gene HDTF1 enhances resistance to Verticillium dahliae and Botrytis cinerea in cotton
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DOI：
10.1111/jipb.12432
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发表时间：
2016-05-01
影响因子：
11.4
通讯作者：
Zhu, Longfu
中科院分区：
文献类型：
--
作者：
Gao, Wei;Long, Lu;Zhu, Longfu
抗病作物的发展，如抗真菌棉花，大大减少了化学施用，提高了作物产量和质量。然而，棉花对大丽花黄萎病等病原菌的抗性机制尚不清楚。在这项研究中，我们鉴定了一个棉花基因（HDTF1），该基因是在棉花对大丽花的抗性反应中通过转录组分析分离出来的。据推测，HDTF1编码一种同源域转录因子，在接种大丽花葡萄球菌和灰葡萄球菌后，HDTF1在棉花中的表达下调。为了描述HDTF1参与对这些病原体的应答，我们使用病毒诱导的基因沉默（VIGS）来产生HDTF1沉默棉花。VIGS降低HDTF1表达显著增强了棉花对这两种病原菌的抗性。HDTF1的沉默导致茉莉酸（JA）介导的信号传导和JA积累的激活。然而，沉默植株在水杨酸（SA）积累和SA信号相关标记基因的表达方面没有改变。这些结果表明，HDTF1是JA通路的负调控因子，可以通过激活JA信号来设计对大丽花和灰绿芽孢杆菌的抗性。
Development of pathogen-resistant crops, such as fungus-resistant cotton, has significantly reduced chemical application and improved crop yield and quality. However, the mechanism of resistance to cotton pathogens such as Verticillium dahliae is still poorly understood. In this study, we characterized a cotton gene (HDTF1) that was isolated following transcriptome profiling during the resistance response of cotton to V. dahliae. HDTF1 putatively encodes a homeodomain transcription factor, and its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea. To characterise the involvement of HDTF1 in the response to these pathogens, we used virus-induced gene silencing (VIGS) to generate HDTF1-silenced cotton. VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens. HDTF1 silencing resulted in activation of jasmonic acid (JA)-mediated signaling and JA accumulation. However, the silenced plants were not altered in the accumulation of salicylic acid (SA) or the expression of marker genes associated with SA signaling. These results suggest that HDTF1 is a negative regulator of the JA pathway, and resistance to V. dahliae and B. cinerea can be engineered by activation of JA signaling.