EFFECT OF SODIUM-HYPOCHLORITE AND GIBBERELLIC-ACID ON SEED DORMANCY AND GERMINATION OF WILD OATS (AVENA-FATUA)
EFFECT OF SODIUM-HYPOCHLORITE AND GIBBERELLIC-ACID ON SEED DORMANCY AND GERMINATION OF WILD OATS (AVENA-FATUA)
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DOI：
10.1139/b79-212
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发表时间：
1979-01-01
期刊：
影响因子：
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通讯作者：
HSIAO, AI
中科院分区：
文献类型：
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作者：
HSIAO, AI
种皮（颖果的果皮、种皮和船体）是野生燕麦（A. fatua L.）。通过将种子浸泡在水中15分钟或更长时间来诱导种子休眠。种子在6%NaOCl中浸泡1 h后再在水中浸泡1 h，休眠诱导率高于仅在水中浸泡1 h的种子。此外，GA 3的种子进行NaOCl处理15分钟或更短的时间内没有打破休眠，表明进入或排除GA 3的种子的速度慢。在GA 3的存在下，发芽增加暴露于NaOCl。通过将干燥种子在NaOCl中浸泡2小时，在水中浸泡1小时，然后在GA 3中孵育种子，获得最大发芽。在NaOCl中浸泡1 h，然后在水中浸泡1 h的吸胀去皮种子完全萌发不需要GA 3;在NaOCl中处理2 h，然后在水中浸泡1 h的完整种子完全萌发需要使用10-4 M GA 3。在这项研究中，吸胀，休眠种子去皮和穿刺需要10-7 GA 3给完全发芽。种子覆盖物的刺穿增强了GA 3的渗透，从而增加了GA 3的发芽率。NaOCl处理种子模拟穿刺的效果。NaOCl也可能导致发芽抑制剂的损失或使这些抑制剂对氧化敏感。延长NaOCl处理导致发芽不良或种子崩解。
The seed coverings, including the pericarp and testa of the caryopsis and the hull, are the main barriers to the exchange of gases and the penetration of exogenous gibberellic acid (GA3) for germination of wild oats (A. fatua L.). Dormancy was induced in seeds by immersing them in water for 15 min or longer. Dormancy induction was greater in seeds immersed for up to 1 h in 6% NaOCl and then 1 h in water than in seeds immersed only in water for 1 h. The addition of GA3 to seeds subjected to NaOCl treatment for 15 min or less did not break dormancy, indicating a slow rate of entry or the exclusion of GA3 by the seeds. In the presence of GA3, germination increased with increasing exposure to NaOCl. Maximum germination was obtained by immersing dry seeds in NaOCl for 2 h, in water for 1 h and then incubating the seeds in GA3. GA3 was not required for complete germination of imbibed, dehulled seeds immersed in NaOCl for 1 h, then in water for 1 h; it was necessary to use 10-4 M GA3 for complete germination of intact seeds that were treated with NaOCl for 2 h, then with water for 1 h. Imbibed, dormant seeds that were dehulled and pierced required 10-7 GA3 to give complete germination in this study. Piercing of the seed coverings enhances GA3 penetration and thus increases the availability of GA3 for germination. NaOCl treatment to the seeds mimics the effects of piercing. NaOCl may also cause loss of germination inhibitors or render these inhibitors susceptible to oxidation. Prolonged NaOCl treatment resulted in either poor germination or seed disintegration.