Laser Printing of Stem Cells for Biofabrication of Scaffold-Free Autologous Grafts
Laser Printing of Stem Cells for Biofabrication of Scaffold-Free Autologous Grafts
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DOI：
10.1089/ten.tec.2010.0359
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发表时间：
2011-01-01
影响因子：
3
通讯作者：
Chichkov, Boris
中科院分区：
文献类型：
--
作者：
Gruene, Martin;Deiwick, Andrea;Chichkov, Boris
干细胞由于其自我更新和分化的能力而在再生医学中受到广泛关注，这是由其三维微环境调节的。在这项研究中，基于激光诱导的前向转移（LIFT）的计算机辅助生物制造技术被用来生成由间充质干细胞（MSC）组成的移植物。我们证明了（i）激光打印不会引起任何细胞损伤;（ii）激光打印的MSC移植物可以向骨和软骨分化;（iii）LIFT允许打印足够高的细胞密度以促进软骨形成;（iv）利用LIFT，可以制造三维无支架自体组织移植物，保持它们的预定结构，和（v）预分化的MSC在整个打印过程中存活并保持其功能性。我们相信，我们的研究结果将在干细胞生物学和组织工程中找到重要的应用。
Stem cells are of widespread interest in regenerative medicine due to their capability of self-renewal and differentiation, which is regulated by their three-dimensional microenvironment. In this study, a computer-aided biofabrication technique based on laser-induced forward transfer (LIFT) is used to generate grafts consisting of mesenchymal stem cells (MSCs). We demonstrate that (i) laser printing does not cause any cell damage; (ii) laser-printed MSC grafts can be differentiated toward bone and cartilage; (iii) LIFT allows printing of cell densities high enough for the promotion of chondrogenesis; (iv) with LIFT three-dimensional scaffold-free autologous tissue grafts can be fabricated keeping their predefined structure, and (v) predifferentiated MSCs survived the complete printing procedure and kept their functionality. We believe that our results will find important applications in stem cell biology and tissue engineering.