Experimental tooth movement into bone induced by recombinant human bone morphogenetic protein-2
Experimental tooth movement into bone induced by recombinant human bone morphogenetic protein-2
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DOI：
10.1597/1545-1569(2003)040
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发表时间：
2003-09-01
期刊：
影响因子：
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通讯作者：
Kuroda, T
中科院分区：
文献类型：
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作者：
Kawamoto, T;Motohashi, N;Kuroda, T
目的：重组人骨形态发生蛋白-2（rhBMP-2）作为牙槽突裂二期植骨的生物材料，在促进骨再生方面具有巨大的潜力。本研究的目的是研究低剂量rhBMP-2（10 μ g/100穆尔）的骨诱导活性和新生成骨对正畸刺激的生物学反应，并与先前使用高剂量rhBMP-2（40 μ g/100穆尔）的结果进行比较。拔出上颌第一前磨牙后，在8只成年比格犬中使用5 mm直径的环锯棒通过手术创建骨缺损。立即植入rhBMP-2和由明胶和聚乳酸聚乙醇酸共聚物制成的PLGA/明胶海绵复合物（PGS）。rhBMP-2的骨诱导活性和牙齿移动到新生成的bone进行了评价的基础上，组织学和形态学检查。结果：戏剧性的组织学和形态学差异被发现在低剂量和高剂量rhBMP-2之间的骨重建的时间过程。低剂量rhBMP-2组骨重建的发生明显提前。在实验性牙齿移动过程中，与高剂量rhBMP-2引起的牙根吸收不同，低剂量rhBMP-2仅引起压力侧牙骨质部分吸收。结论：rhBMP-2的临床应用需要确定合适的剂量。
Objective: The development of biomaterials for secondary bone grafting to the alveolar cleft, recombinant human morphogenetic protein-2 (rhBMP-2) may have great potential to promote osseous regeneration. The purpose of this study was to investigate the osteoinductive activity of low-dose rhBMP-2 (10 mug/100 muL) and the biological response of newly generated bone to orthodontic stimulation, compared with previous results using high-dose rhBMP-2 (40 mug/100 muL).Method. After extraction of the maxillary first premolars, bone defects were surgically created in eight adult beagle dogs using a 5-mm-diameter trepan bar. The rhBMP-2 with a PLGA/gelatin sponge complex (PGS) made of gelatin and polylactic acid polyglycolic acid copolymer was immediately implanted. The osteoinductive activity of rhBMP-2 and tooth movement into the newly generated bone was evaluated based on histological and morphometric examination.Results: Dramatic histological and morphometric differences were found in the time course of bone remodeling between low- and high-dose rhBMP-2. The onset of bone remodeling occurred significantly earlier in low-dose rhBMP-2. During experimental tooth movement, in contrast to high-dose rhBMP-2, with which root resorption occurred, only partial cementum resorption occurred on the pressure side with low-dose rhBMP-2.Conclusion: These results indicated that the proper dosage of rhBMP-2 should be determined for successful clinical application.