Mutagenicities of 8-hydroxyguanine and 2-hydroxyadenine produced by reactive oxygen species
Mutagenicities of 8-hydroxyguanine and 2-hydroxyadenine produced by reactive oxygen species
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DOI：
10.1248/bpb.27.475
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发表时间：
2004-04-01
影响因子：
2
通讯作者：
Kamiya, H
中科院分区：
文献类型：
--
作者：
Kamiya, H
合成含有 8-羟基鸟嘌呤和 2-羟基腺嘌呤（由活性氧在细胞中产生的嘌呤损伤）的寡脱氧核糖核苷酸并将其插入载体 DNA 中，以在预定位点引入每个损伤。将处理过的 DNA 转染到活细胞中，收集并分析每个 DNA 损伤诱导的突变体。此外，还通过使用化学合成的三磷酸核苷研究了具有两个氧化嘌呤碱基的受损DNA前体诱导的突变。在这篇综述文章中，作者通过重点关注作者及其合作者检查的实验，总结了两种氧化嘌呤碱基的致突变潜力。
Oligodeoxyribonucleotides containing 8-hydroxyguanine and 2-hydroxyadenine, purine lesions produced in cells by reactive oxygen species, were synthesized and inserted into vector DNAs to introduce each lesion at a predetermined site. The manipulated DNAs were transfected into living cells, and the mutants induced by each DNA lesion were collected and analyzed. In addition, the mutations induced by damaged DNA precursors with the two oxidized purine bases were studied by the use of chemically synthesized nucleoside triphosphates. In this review article, the author summarizes the mutagenic potentials of the two oxidized purine bases, by focusing on experiments examined by the author and his collaborators.