The effects of ovarian hormones on stressor-induced hormonal responses, glucocorticoid receptor expression and translocation, and genes related to receptor signaling in adult female rats
The effects of ovarian hormones on stressor-induced hormonal responses, glucocorticoid receptor expression and translocation, and genes related to receptor signaling in adult female rats
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卵巢激素对成年雌性大鼠应激诱导的激素反应、糖皮质激素受体表达和易位以及受体信号相关基因的影响
作者：
Matthew R. Green;M. Marcolin;C. McCormick
雌二醇增强雌性大鼠应激源暴露后下丘脑-垂体-肾上腺活性并延迟糖皮质激素分泌恢复到基线水平；我们研究了雌二醇的作用是否涉及糖皮质激素受体（GR）易位和受体共伴侣的表达。在实验1中，完整雌性和去卵巢雌性分别用载药（VEH）、17β-雌二醇苯甲酸酯（EB）或EB和黄体酮（EB + P）处理4天。样本取自家鼠笼（基线）或塑料约束器约束30分钟后（约束后）的大鼠（n = 10/组）。与所有其他治疗相比，OVX-VEH治疗降低了基线和约束后血浆皮质酮浓度。Western blot结果显示，OVX-VEH处理大鼠海马胞浆GR表达明显高于其他处理。应激使海马核GR表达增加，但无处理差异。实验2:OVX大鼠每天分别给予VEH、EB或EB + P治疗（n = 8/组）。抑制应激对OVX-VEH大鼠皮质酮分泌的刺激低于其他处理，OVX-EB + P处理大鼠皮质酮浓度低于OVX-EB组，提示黄体酮减轻了雌二醇的作用。定量聚合酶链反应实验表明，应激增加了海马腹侧Fkbp5 mRNA的表达，应激或处理对Nr3c1 （GR）、Nr3c2 （MR）、Fkbp4、Bag1、Ncoa1 （SRC-1）表达无影响。因此，关于雌二醇对负反馈的影响是由海马中受体共伴侣或共调节剂的表达改变介导的这一假设不被支持。雌二醇可能通过限制胞质GR蛋白的可用性来减弱反馈。
Abstract Estradiol potentiates hypothalamic-pituitary-adrenal activity and delays the return of glucocorticoid secretion to baseline after a stressor exposure in female rats; we investigated whether estradiol effects involve actions on glucocorticoid receptor (GR) translocation and expression of receptor co-chaperones. In Experiment 1 intact females and ovariectomized (OVX) females were treated for four days with vehicle (VEH), 17β-estradiol benzoate (EB), or EB and progesterone (EB + P). Samples were taken from rats in the home cage (baseline) or after 30 min of restraint stress in a plastic restrainer (post-restraint) (n = 10/group). OVX-VEH treatment reduced baseline and post-restraint plasma concentrations of corticosterone versus all other treatments. Western blots indicated that OVX-VEH treated rats had greater hippocampal cytosolic GR expression than other treatments. Stress increased hippocampal nuclear GR expression, but without treatment differences. In Experiment 2 OVX rats were treated daily with VEH, EB, or EB + P (n = 8/group). OVX-VEH rats showed a lower stimulation of corticosterone secretion by restraint stress than other treatments and OVX-EB + P treated rats had lower concentrations than the OVX-EB group, suggesting progesterone mitigated estradiol effects. Quantitative polymerase chain reaction experiments indicated that stress increased Fkbp5 mRNA in the ventral hippocampus, with no effect of stress or treatment on Nr3c1 (GR), Nr3c2 (MR), Fkbp4, Bag1, or Ncoa1 (SRC-1) expression. Thus, the hypothesis is that estradiol effects on negative feedback are mediated by altered expression of receptor co-chaperones or co-modulators in the hippocampus was not supported. Estradiol may blunt feedback by limiting the availability of cytosolic GR protein.
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去势大鼠中 ACTH 分泌的快速糖皮质激素反馈抑制：慢性雌激素和孕激素的作用。
影响因子：
4.1
作者：
Redei,E;Li,L;Halasz,I;McGivern,RF;Aird,F
通讯作者：
Aird,F
DOI：
10.1152/ajpendo.00102.2006
发表时间：
2007-04-01
影响因子：
5.1
作者：
Figueiredo, Helmer F.;Ulrich-Lai, Yvonne M.;Herman, James P.
通讯作者：
Herman, James P.
影响因子：
6.1
作者：
B. B. Turner-B.
通讯作者：
B. B. Turner-B.
影响因子：
13.5
作者：
Hapgood, Janet P.;Avenant, Chanel;Moliki, Johnson M.
通讯作者：
Moliki, Johnson M.
大鼠脑中雌激素受体免疫反应性：雌二醇注射的快速作用。
影响因子：
4.8
作者：
Blaustein,JD
通讯作者：
Blaustein,JD