POTENTIAL ROLE OF MITOGEN-ACTIVATED PROTEIN-KINASE IN PRONUCLEAR ENVELOPE ASSEMBLY AND DISASSEMBLY FOLLOWING FERTILIZATION OF MOUSE EGGS
POTENTIAL ROLE OF MITOGEN-ACTIVATED PROTEIN-KINASE IN PRONUCLEAR ENVELOPE ASSEMBLY AND DISASSEMBLY FOLLOWING FERTILIZATION OF MOUSE EGGS
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DOI：
10.1095/biolreprod53.3.692
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发表时间：
1995-09-01
影响因子：
3.6
通讯作者：
KOPF, GS
中科院分区：
文献类型：
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作者：
MOOS, J;VISCONTI, PE;KOPF, GS
用体外受精的小鼠卵细胞分析了p34（cdc 2）/cyclin B复合物和丝裂原活化蛋白（MAP）激酶活性的变化。（p34（cdc 2）/细胞周期蛋白B）在授精后90分钟下降到可忽略的水平，髓鞘碱性蛋白激酶活性下降到可忽略的水平（即MAP激酶）直到授精后约7小时才被观察到。MAP激酶活性的降低似乎与p34（cdc 2）/细胞周期蛋白B激酶活性的先前降低有关，因为通过用微管抑制剂诺考达唑处理卵子来抑制受精诱导的细胞周期蛋白B的破坏，防止了这些蛋白激酶中每一种的降低;完整的纺锤体是细胞周期蛋白破坏所必需的，此外，在维持可忽略水平的p34（cdc 2）/细胞周期蛋白B激酶活性的条件下，通过冈田酸处理MAP激酶活性的实验性升高提示MAP激酶可能参与原核包膜动力学。具体而言，防止受精诱导的MAP激酶活性降低与抑制原核形成相关，原核形成后MAP激酶活性升高导致早熟原核包膜进入M期之前的破裂。
Changes in the activities of the p34(cdc2)/cyclin B complex and mitogen-activated protein (MAP) kinase were analyzed after insemination of mouse eggs in vitro, Whereas histone H1 kinase activity (p34(cdc2)/cyclin B) fell to negligible levels by 90 min postinsemination, a decrease to negligible levels of myelin basic protein kinase activity (i.e,, MAP kinase) was not observed until about 7 h postinsemination. The decrease in MAP kinase activity appeared to be linked to the prior decline in p34(cdc2)/cyclin B kinase activity, since inhibiting the fertilization-induced destruction of cyclin B by treating eggs with the microtubule inhibitor nocodazole prevented the decrease in each of these protein kinases; an intact spindle is required for cyclin destruction, Moreover, experimental elevation of MAP kinase activity by okadaic acid treatment under conditions that maintain negligible levels of p34(cdc2)/cyclin B kinase activity suggested that MAP kinase could be involved in pronuclear envelope dynamics. Specifically, preventing the fertilization-induced decrease in MAP kinase activity was correlated with inhibiting pronucleus formation, and elevating MAP kinase activity subsequent to pronucleus formation resulted in precocious pronuclear envelope breakdown prior to entry into M phase.