Conventional optical microscopy of colloidal suspensions.
Conventional optical microscopy of colloidal suspensions.
复制标题
胶体悬浮液的传统光学显微镜。
DOI：
10.1016/s0001-8686(00)00070-1
复制
发表时间：
2001
影响因子：
15.6
通讯作者：
W. Poon
中科院分区：
文献类型：
--
作者：
M. S. Elliot;W. Poon
光学显微镜可以解决细节在较大的一端的胶体长度尺度，并在一个单独的颗粒水平的分辨率图像悬浮液将允许调查的方式拒绝建立散射技术的本地行为。然而，要实现高对比度的单粒子分辨率在稠密的悬浮液，是足够厚的表现出相同的行为，预计在散装不是一个微不足道的练习。我们建立在建立先进的技术，传统的（即非共焦）光学显微镜的相位对象，以开发一个合适的实验协议。此外，我们证明了该协议的有效性的硬球晶体固体（随机堆叠的结果在许多复杂的方面），这应该作为一个纲要，为今后的研究的“图集”的手段。
Optical microscopy can resolve detail at the larger end of the colloidal length scale, and to image suspensions at an individual particle level of resolution would allow the investigation of local behaviour in a way denied to the established scattering techniques. However, to achieve high-contrast single-particle resolution in dense suspensions that are thick enough to show behaviour the same as would be expected in the bulk is not a trivial exercise. We build on established advanced techniques of the conventional (i.e. non-confocal) light microscopy of phase objects to develop a suitable experimental protocol. Furthermore, we demonstrate the effectiveness of this protocol by means of an ‘atlas’ of the hard-sphere crystalline solid (where random stacking results in many complex facets), which should serve as a compendium for future study.