DogMATIC-A Remote Biospecimen Collection Kit for Biobanking
DogMATIC-A Remote Biospecimen Collection Kit for Biobanking
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DOI：
10.1089/bio.2014.0085
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发表时间：
2015-08-01
影响因子：
1.6
通讯作者：
Danks, Janine A.
中科院分区：
文献类型：
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作者：
Milley, Kristi M.;Nimmo, Judith S.;Danks, Janine A.
犬肿瘤是有价值的比较肿瘤学模型。这项研究旨在为乳腺癌研究创建一个可持续的犬乳腺肿瘤生物库。其目的是为样本共享、数据挖掘和长期研究目标提供一个具有良好特征的样本队列。犬类乳腺肿瘤最常在当地的兽医诊所或医院进行治疗。我们采用了基于大量参与的兽医医院和诊所作为收集中心的生物库框架，这些医院和诊所由中央存储设施提供服务。招募的目标是农村兽医诊所。设计了一种量身定做的、稳定的收集盒(教条)，供偏远或农村地区的兽医用来收集新鲜和固定的组织，以便提交给生物库。为了验证该方法，对试剂盒设计、采集率和样品质量进行了分析。澳大利亚兽医癌症生物库是由47个兽医诊所和三个兽医病理实验室组成的网络，覆盖范围超过200,000公里(2)。在最初的12个月里，30例犬乳腺肿瘤病例是通过Dogic试剂盒提交的。在80%以上的样品中提取到了完整的纯RNA，平均产量为14.49微克。成功地协调了一个大型网络生物库，该生物库利用DIOMIC试剂盒进行异地收集。澳大利亚兽医癌症生物库的建立在澳大利亚建立了一个长期、可持续、可比较的肿瘤学研究资源。这种截然不同的收集和银行业务形式，对生物银行业务有着更广泛的影响。
Canine tumors are valuable comparative oncology models. This research was designed to create a sustainable biobank of canine mammary tumors for breast cancer research. The aim was to provide a well-characterized sample cohort for specimen sharing, data mining, and long-term research aims. Canine mammary tumors are most frequently managed at a local veterinary clinic or hospital. We adopted a biobank framework based on a large number of participating veterinary hospitals and clinics acting as collection centers that were serviced by a centralized storage facility. Recruitment was targeted at rural veterinary clinics. A tailored, stable collection kit (DogMATIC) was designed that was used by veterinarians in remote or rural locations to collect both fresh and fixed tissue for submission to the biobank. To validate this methodology the kit design, collection rate, and sample quality were analyzed. The Australian Veterinary Cancer Biobank was established as a network of 47 veterinary clinics and three veterinary pathology laboratories spanning over 200,000 km(2). In the first 12 months, 30 canine mammary tumor cases were submitted via the DogMATIC kit. Pure intact RNA was isolated in over 80% of samples with an average yield of 14.49 mu g. A large network biobank, utilizing off-site collection with the DogMATIC kit, was successfully coordinated. The creation of the Australian Veterinary Cancer Biobank has established a long-term, sustainable, comparative oncology research resource in Australia. There are broader implications for biobanking with this very different form of collection and banking.