Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal polycomb sites
Drosophila enhancer of Zeste/ESC complexes have a histone H3 methyltransferase activity that marks chromosomal polycomb sites
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DOI：
10.1016/s0092-8674(02)00975-3
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发表时间：
2002-10-18
期刊：
影响因子：
64.5
通讯作者：
Pirrotta, V
中科院分区：
文献类型：
--
作者：
Czermin, B;Melfi, R;Pirrotta, V
Zust的增强子是一种多梳状蛋白，对建立和维持同源异型基因和其他基因的抑制是必不可少的。在早期胚胎中，它被发现存在于包括ESC的复合体中，并被招募到Polycomb反应元件中。我们发现该复合体含有甲基化转移酶活性，可以甲基化组蛋白H3的赖氨酸9和27，但当E(Z)SET结构域发生突变时，该活性就会丧失。赖氨酸9位是三甲基化的，这一标记与多线染色体上的多梳结合位点密切相关，但也存在于中心异染色质、4号染色体和端粒位点。由E(Z)/ESC复合体体外甲基化的组蛋白H3与Polycomb蛋白特异性结合。
Enhancer of Zeste is a Polycomb Group protein essential for the establishment and maintenance of repression of homeotic and other genes. In the early embryo it is found in a complex that includes ESC and is recruited to Polycomb Response Elements. We show that this complex contains a methyltransferase activity that methylates lysine 9 and lysine 27 of histone H3, but the activity is lost when the E(Z) SET domain is mutated. The lysine 9 position is trimethylated and this mark is closely associated with Polycomb binding sites on polytene chromosomes but is also found in centric heterochromatin, chromosome 4, and telomeric sites. Histone H3 methylated in vitro by the E(Z)/ ESC complex binds specifically to Polycomb protein.