RYANODINE RECEPTOR OF SKELETAL-MUSCLE IS A GAP JUNCTION TYPE CHANNEL
RYANODINE RECEPTOR OF SKELETAL-MUSCLE IS A GAP JUNCTION TYPE CHANNEL
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DOI：
10.1126/science.2459777
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发表时间：
1988-10-07
期刊：
影响因子：
56.9
通讯作者：
CORONADO, R
中科院分区：
文献类型：
--
作者：
MA, J;FILL, M;CORONADO, R
在骨骼肌的肌浆网膜中，兰尼碱受体形成一个水孔，被鉴定为在兴奋-收缩偶联过程中起作用的钙释放途径。纯化的兰尼碱受体通道目前已被证明具有通常与间隙连接通道相关的四种性质：（i）由几种开放状态组成的大的非特异性电压依赖性电导;（ii）低pH抑制开放概率;（iii）钙抑制开放概率;（iv）对庚醇和辛醇（而不是其他醇）阻断的敏感性。这种功能上的同源性可以提供一个深入了解的机制，肌肉细胞如何去极化成细胞内释放钙。
In the sarcoplasmic reticulum membrane of skeletal muscle, the ryanodine receptor forms an aqueous pore identified as the calcium-release pathway that operates during excitation-contraction coupling. The purified ryanodine receptor channel has now been shown to have four properties usually associated with gap junction channels: (i) a large nonspecific voltage-dependent conductance consisting of several open states; (ii) an inhibition of open probability by low pH; (iii) an inhibition of open probability by calcium; and (iv) a sensitivity to blockade by heptanol and octanol but not other alcohols. This functional homology may provide an insight into the mechanism of how muscle cells transduce depolarization into an intracellular release of calcium.