Uptake of some tritiated sympathomimetic amines by mouse brain cortex slices in vitro.
Uptake of some tritiated sympathomimetic amines by mouse brain cortex slices in vitro.
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体外小鼠大脑皮层切片对一些氚拟交感胺的摄取。
DOI：
10.1111/j.1600-0773.1966.tb00392.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
A. L. Renyi
中科院分区：
文献类型：
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作者：
S. Ross;A. L. Renyi
方法按Ross & RENYI 1964所述，将氚化胺与小鼠脑皮质组织一起孵育。孵育混合物含有：约100 mg脑切片、0.2 mpmol研究中的氚化胺、2 ml Krebs-Henseleit溶液和待测抑制剂。孵育的环境为93.5%O2和6.5%CO2。当使用短孵育时间时，在添加胺之前将切片在37 ° C下预孵育5分钟。用乙醇提取组织，并如前所述在液体闪烁系统中记录放射性（Ross & RENYI 1964）。该量表示为每克组织（湿重）的胺的mpmol。在某些情况下，根据先前使用的公式（Ross & RENYI），将摄取表示为切片中的放射性浓度与孵育溶液中的放射性浓度的比率以及摄取的抑制百分比
MethodsThe tritiated amines were incubated with the cortex tissue from mouse brain as described previously (Ross & RENYI 1964). The incubation mixture contained: about 100 mg of brain slices, 0.2 mpmol of the tritiated amine under investigation, 2 ml of Krebs-Henseleit solution and the inhibitor to be tested. The incubation athmosphere was 93.5% 02 and 6.5% COz. When short incubation times were used, the slices were pre-incubated at 37" for five minutes before the addition of the amine. The tissues were extracted with ethanol and the radioactivities recorded in a liquid scintillation system as previously described (Ross & RENYI 1964).The radioactivity in the tissue was taken as a measure of the amount of the amine taken up by the tissue. This amount was expressed as mpmol of the amine per gram tissue (wet weight). In some cases, the uptake was expressed as the ratio of the concentration of the radioactivity in the slices to that in the incubation solution and the percentage inhibition of the uptake according to the formula previously used (Ross & RENYI