Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood.
Protocol for generating human induced neural progenitor cells from immobilized adult peripheral blood.
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从固定的成人外周血中产生人类诱导神经祖细胞的方案。
DOI：
10.1016/j.xpro.2021.100346
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Jing N
中科院分区：
文献类型：
--
作者：
Zhang T;Tao R;Yue C;Jing N
从体细胞产生用于医学应用的诱导神经干/祖细胞（iNPC）仍然具有挑战性。在这里，我们描述了一个可靠的协议，使人iNPC从一个小体积的固定成人外周血直接重编程。我们已经证实，无整合的人iNPC在移植后可以有效地分化为小鼠脑中的成熟神经元，并显示出功能性替代受损神经元的能力，这表明它们在开发神经退行性疾病的替代药物中作为供体细胞的潜力。有关本方案使用和执行的完整详细信息，请参阅。从成人外周血产生人iNPC的可靠方案建立人iNPC系的详细程序系统表征人iNPC和iNPC系的方法有效指导人iNPC的神经元分化的方法从体细胞产生用于医学应用的诱导神经干/祖细胞（iNPC）仍然具有挑战性。在这里，我们描述了一个可靠的协议，使人iNPC从一个小体积的固定成人外周血直接重编程。我们已经证实，无整合的人iNPC在移植后可以有效地分化为小鼠脑中的成熟神经元，并显示出功能性替代受损神经元的能力，这表明它们在开发神经退行性疾病的替代药物中作为供体细胞的潜力。
Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases. For complete details on the use and execution of this protocol, please refer to. A reliable protocol to generate human iNPCs from adult peripheral blood Detailed procedures for establishment of human iNPC lines Approach to systematically characterize human iNPCs and iNPC lines Method for efficiently directing the neuronal differentiation of human iNPCs Generating induced neural stem/progenitor cells (iNPCs) from somatic cells for medical applications has remained challenging. Here, we describe a reliable protocol to make human iNPCs from a small volume of immobilized adult peripheral blood by direct reprogramming. We have verified that the integration-free human iNPCs can efficiently differentiate into mature neurons in mouse brain upon transplantation and display capacities to functionally replace the damaged neurons, suggesting their potential as donor cells in developing replacement medicine for neurodegenerative diseases.
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影响因子：
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通讯作者：
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