Cold exposure, gut microbiota, and hypertension: A mechanistic study
Cold exposure, gut microbiota, and hypertension: A mechanistic study
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寒冷暴露、肠道微生物群和高血压：一项机制研究
DOI：
10.1016/j.scitotenv.2022.155199
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发表时间：
2022-04-19
影响因子：
9.8
通讯作者：
Luo, Bin
中科院分区：
文献类型：
--
作者：
Wang, Bo;Liu, Jiangtao;Luo, Bin
寒冷暴露已被认为是高血压的重要危险因素，据报道，肠道微生物群的改变与高血压有关。我们假设肠道微生物群与冷诱导高血压（CIH）之间存在合理的关系。因此，我们探索了肠道微生物群及其代谢产物与CIH之间的潜在联系。将雄性Sprague-Dawley（SD）大鼠随机分为常温组（NT，20 ± 2 ℃）和冷暴露组（CE，4 ± 1 ℃），6周后进行粪便细菌交叉移植。我们使用16 S rDNA序列分析了大鼠的肠道菌群，并测量了大鼠的血压和大鼠粪便中短链脂肪酸的含量。冷暴露6周后，成功建立CIH大鼠模型。冷暴露降低了肠道微生物群的多样性，增加了潜在致病性和条件致病性细菌（例如，Quinella、Rothia和Senegalimassilia属），并减少了有益细菌（例如，乳杆菌属）和丁酸产生细菌（例如，毛螺菌科UCG-008和瘤胃球菌科UCG-013属）。粪便细菌交叉移植改变了肠道微生物群组成并调节了血压水平。移植CIH大鼠粪便细菌的NT组大鼠富含某些条件致病菌，如普雷沃氏菌UCG-003属。移植了来自NT组大鼠的粪便细菌的CIH大鼠富含有益细菌，例如拟杆菌属。此外，我们发现CIH大鼠的丁酸水平显著降低，这可能与血压升高有关。总之，CIH与肠道微生物群的改变和丁酸的减少有关。我们的研究结果通过补充有益细菌/丁酸盐来调节肠道微生物群，为CIH的预防和治疗提供了新的见解。
Cold exposure has been recognized as an important risk factor for hypertension, and altered gut microbiota has been reported to be associated with hypertension. We hypothesized that there is a plausible relationship between gut micro biota and cold-induced hypertension (CIH). Therefore, we explored the potential link between the gut microbiota and its metabolites with CIH. Male Sprague-Dawley (SD) rats were randomly divided into the normal temperature group (NT, 20 +/- 2 degrees C) and the cold exposure group (CE, 4 +/- 1 degrees C), and faecal bacteria cross-transplantation was performed after six weeks. We analyzed the gut microbiota of rats using the 16S rDNA sequence and measured the blood pressure of rats and the content of short-chain fatty acids in rat faeces. After six weeks of cold exposure, the CIH rat model was successfully established. The cold exposure reduced the diversity of the gut microbiota, increased the abundance of potentially pathogenic and conditionally pathogenic bacteria (e.g., Quinella, Rothia, and Senegalimassilia genera), and reduced the abundance of beneficial bacteria (e.g., Lactobacillus genus) and butyric acid-producing bacteria (e.g., Lachnospiraceae UCG-008 and Ruminococcaceae UCG-013 genera). Faecal bacteria cross-transplantation altered gut microbiota composition and regulated blood pressure levels. The NT group rats transplanted with CIH rats' faecal bacteria were enriched with certain conditional pathogenic bacteria such as Prevotellaceae UCG-003 genus. The CIH rats transplanted with faecal bacteria from the NT group rats were enriched with beneficial bacteria such as Bacteroides genus. In addition, we found a significant reduction in butyric acid levels in CIH rats, which may be related to the increase in blood pressure. In conclusion, CIH is associated with altered gut microbiota and reduced butyric acid. Our findings provide novel insights for the prevention and treatment of CIH by modulating the gut microbiota through supplementation of beneficial bacteria/butyrate.