Measurement of Airborne Bacteria and Endotoxin Generated During Dental Cleaning
Measurement of Airborne Bacteria and Endotoxin Generated During Dental Cleaning
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DOI：
10.1080/15459620802633957
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发表时间：
2009-01-01
影响因子：
2
通讯作者：
Duchaine, Caroline
中科院分区：
文献类型：
--
作者：
Dutil, Steve;Meriaux, Anne;Duchaine, Caroline
动态牙科器械在工作环境中会产生大量的气雾剂。牙科单位水线(DUWL)支持大量微生物种群，并可能是牙科治疗过程中产生生物气溶胶的重要来源。这项研究是为了表征在标准化条件下进行的牙科治疗过程中生物气溶胶的产生。采用培养法(R2A法，血琼脂加氧和不加氧)和荧光显微镜观察。牙齿清洁程序是在一个通风速率可控的隔离治疗室进行的。使用安徒生微生物采样器收集在牙科治疗前(基线)、治疗期间和治疗2小时后产生的可培养生物气溶胶。使用可吸入粉尘采样器测量口腔卫生员和患者呼吸区的总生物气溶胶含量。用AgI-30收集内毒素。荧光显微镜结合培养的使用表明，牙科工作人员和患者暴露在治疗过程中产生的高达1.86E+05细菌/立方米的环境中。幸运的是，生物气雾剂在牙科手术后2小时内恢复到基线水平。产生的气雾剂直径很小(1微米)，这表明气雾化细菌很可能与接触者的呼吸系统接触。
Dynamic dental instruments generate abundant aerosols in the work environment. Dental unit waterlines (DUWL) support a large microbial population and can be a significant source of bioaerosols generated during dental treatments. This study was conducted to characterize bioaerosol generation during dental treatments performed in standardized conditions. Culture-based method (R2A, and blood agar with and without O-2) and fluorescence microscopy were used. Dental cleaning procedures were performed in an isolated treatment room with controlled ventilation rate. Andersen microbial samplers were used to collect culturable bioaerosols generated before ( baseline), during, and after 2 hr of dental treatments. Inhalable dust samplers were used to measure total bioaerosols content in dental hygienist's and patients' breathing zones. AGI-30 were used for the collection of the endotoxin. The use of fluorescence microscopy in combination with culture demonstrated that dental staff and patients were exposed to up to 1.86 E+05 bacteria/m(3) generated during treatments. Fortunately, bioaerosols returned to baseline within 2 hr after the dental procedures. The small diameter of the aerosols generated (< 1 mu m) suggests that the risk of contact between the aerosolized bacteria and the respiratory system of exposed individuals is likely to occur.