Nano/Micro Formulations for Bacteriophage Delivery
Nano/Micro Formulations for Bacteriophage Delivery
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DOI：
10.1007/978-1-4939-7395-8_20
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发表时间：
2018-01-01
期刊：
影响因子：
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通讯作者：
Llagostera, Montserrat
中科院分区：
文献类型：
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作者：
Cortes, Pilar;Cano-Sarabia, Mary;Llagostera, Montserrat
包裹方法允许保护噬菌体以克服关键环境条件。此外，它们还提高了噬菌体的稳定性和受控递送，这在噬菌体治疗中具有重大的创新价值。在这里，使用两种生物相容材料描述了两种不同的包裹噬菌体的方法：脂类阳离子混合物和海藻酸盐与抗酸剂CaCO3的组合。要进行噬菌体包裹，需要高浓度(约10(10)-10(11)pfu/mL)的纯化裂解液，并处置特定的设备。这两种方法都已成功地应用于不同形态的沙门氏菌噬菌体的包埋。此外，所使用的材料不会改变噬菌体的抗菌作用。此外，这两种技术还可以适用于任何噬菌体，并可能适用于任何噬菌体疗法的递送途径。
Encapsulation methodologies allow the protection of bacteriophages for overcoming critical environmental conditions. Moreover, they improve the stability and the controlled delivery of bacteriophages which is of great innovative value in bacteriophage therapy. Here, two different encapsulation methodologies of bacteriophages are described using two biocompatible materials: a lipid cationic mixture and a combination of alginate with the antacid CaCO3. To perform bacteriophage encapsulation, a purified lysate highly concentrated (around 10(10)-10(11) pfu/mL) is necessary, and to dispose of a specific equipment. Both methodologies have been successfully applied for encapsulating Salmonella bacteriophages with different morphologies. Also, the material employed does not modify the antibacterial action of bacteriophages. Moreover, both technologies can also be adapted to any bacteriophage and possibly to any delivery route for bacteriophage therapy.