Plastic compressed collagen as a novel carrier for expanded human corneal endothelial cells for transplantation.
Plastic compressed collagen as a novel carrier for expanded human corneal endothelial cells for transplantation.
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塑料压缩胶原作为用于移植的扩大的人角膜内皮细胞的新型载体。
DOI：
10.1371/journal.pone.0050993
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Daniels JT
中科院分区：
文献类型：
--
作者：
Levis HJ;Peh GS;Toh KP;Poh R;Shortt AJ;Drake RA;Mehta JS;Daniels JT
目前治疗角膜内皮细胞衰竭引起的可逆性失明的方法包括使用一个供体-一个受体的策略，用供体组织替换失败的内皮细胞。由于世界范围内供体角膜短缺的压力越来越大，人们对开发使用扩展细胞替代疗法治疗内皮功能障碍的替代策略有相当大的兴趣。已经开发出了允许成功地在体外扩增内皮细胞的方案，但这种方法需要一种支持材料，使细胞能够容易地转移到接受者。我们描述了塑料压缩胶原作为一种高效的、新型的人角膜内皮细胞载体的首次使用。人角膜内皮细胞株和原代人角膜内皮细胞在胶原支架上培养14d后仍保持其鹅卵石样形态和紧密连接蛋白ZO-1和泵蛋白Na+/K+ATPaseα1的表达。此外，超微结构分析显示内皮层紧密结合，细胞和顶端微绒毛紧密相对。塑料压缩胶原蛋白是一种优越的生物材料，因为它的生产速度和简单性，以及它能够以临床相关的方式进行操作而不会损坏。这种方法为扩张的内皮细胞提供了一种适合移植的底物，允许一个供体角膜潜在地治疗多名患者。
Current treatments for reversible blindness caused by corneal endothelial cell failure involve replacing the failed endothelium with donor tissue using a one donor-one recipient strategy. Due to the increasing pressure of a worldwide donor cornea shortage there has been considerable interest in developing alternative strategies to treat endothelial disorders using expanded cell replacement therapy. Protocols have been developed which allow successful expansion of endothelial cells in vitro but this approach requires a supporting material that would allow easy transfer of cells to the recipient. We describe the first use of plastic compressed collagen as a highly effective, novel carrier for human corneal endothelial cells. A human corneal endothelial cell line and primary human corneal endothelial cells retained their characteristic cobblestone morphology and expression of tight junction protein ZO-1 and pump protein Na+/K+ ATPase α1 after culture on collagen constructs for up to 14 days. Additionally, ultrastructural analysis suggested a well-integrated endothelial layer with tightly opposed cells and apical microvilli. Plastic compressed collagen is a superior biomaterial in terms of its speed and ease of production and its ability to be manipulated in a clinically relevant manner without breakage. This method provides expanded endothelial cells with a substrate that could be suitable for transplantation allowing one donor cornea to potentially treat multiple patients.
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影响因子：
3.7
作者：
Peh GS;Toh KP;Wu FY;Tan DT;Mehta JS
通讯作者：
Mehta JS
影响因子：
2
作者：
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影响因子：
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作者：
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影响因子：
13.7
作者：
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通讯作者：
Tsubota, K