NG2 proteoglycan promotes endothelial cell motility and angiogenesis via engagement of galectin-3 and α3β1 integrin
NG2 proteoglycan promotes endothelial cell motility and angiogenesis via engagement of galectin-3 and α3β1 integrin
复制标题
DOI：
10.1091/mbc.e04-03-0236
复制
发表时间：
2004-08-01
影响因子：
3.3
通讯作者：
Stallcup, WB
中科院分区：
文献类型：
--
作者：
Fukushi, J;Makagiansar, IT;Stallcup, WB
NG2蛋白多糖由新生血管中的微血管周细胞表达。我们使用体外和体内模型来研究NG2在周细胞和内皮细胞(EC)之间的串扰中的作用。可溶性NG2与内皮细胞表面的结合在体外诱导细胞运动和多细胞网络的形成，并在体内刺激角膜血管生成。生化数据表明Galectin-3和Alpha3beta1整合素都参与了EC对NG2的反应，并且NG2、Galectin-3和Alpha3beta1在细胞表面形成了一个复合体。在该系统中，通过α3beta1的跨膜信号参与了EC的运动和形态发生。Galectin-3依赖的寡聚作用可能增强NG2介导的α3β1的激活。结合最近的研究表明周细胞早期参与了血管生成，这些数据表明周细胞衍生的NG2在新生血管形成的早期阶段是促进EC迁移和形态形成的重要因素。
The NG2 proteoglycan is expressed by microvascular pericytes in newly formed blood vessels. We have used in vitro and in vivo models to investigate the role of NG2 in cross-talk between pericytes and endothelial cells (EC). Binding of soluble NG2 to the EC surface induces cell motility and multicellular network formation in vitro and stimulates corneal angiogenesis in vivo. Biochemical data demonstrate the involvement of both galectin-3 and alpha3beta1 integrin in the EC response to NG2 and show that NG2, galectin-3, and alpha3beta1 form a complex on the cell surface. Transmembrane signaling via alpha3beta1 is responsible for EC motility and morphogenesis in this system. Galectin-3- dependent oligomrization may potentiate NG2-mediated activation of alpha3beta1. In conjunction with recent studies demonstrating the early involvement of pericytes in angiogenesis, these data suggest that pericyte-derived NG2 is an important factor in promoting EC migration and morphogenesis during the early stages of neovascularization.