RECOMBINANT FOWLPOX VIRUS INDUCING PROTECTIVE IMMUNITY IN NON-AVIAN SPECIES
RECOMBINANT FOWLPOX VIRUS INDUCING PROTECTIVE IMMUNITY IN NON-AVIAN SPECIES
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DOI：
10.1016/0264-410x(88)90100-4
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发表时间：
1988-12-01
期刊：
影响因子：
5.5
通讯作者：
PAOLETTI, E
中科院分区：
文献类型：
--
作者：
TAYLOR, J;WEINBERG, R;PAOLETTI, E
鸡痘病毒的自然宿主仅限于禽类。然而，当接种到非禽类组织培养细胞中时，鸡痘病毒可以引发流产感染。设计了一种表达狂犬病病毒糖蛋白的鸡痘病毒。在将重组病毒接种到禽类(许可)或非禽类(非许可)细胞中时，狂犬病糖蛋白被表达为膜相关抗原。将重组的流痘病毒接种到6种不同种类的哺乳动物体内，可产生对流痘抗原和狂犬病糖蛋白的特异性免疫反应。在小鼠、猫和狗身上，免疫反应足以抵御活的狂犬病病毒的挑战。这一结果证明了在缺乏鸡痘病毒载体的有效病毒复制的情况下，鸡痘病毒载体在非禽类物种中免疫狂犬病的有效性。
The natural host of fowlpox virus is limited to avian species. When inoculated into non-avian tissue culture cells, however, fowlpox virus can initiate an abortive infection. A fowlpox virus was engineered to express rabies virus glycoprotein. On inoculation of the recombinant virus into either avian (permissive) or non-avian (non-permissive) cells, the rabies glycoprotein was expressed as a membrane-associated antigen. Inoculation of the flowlpox virus recombinant into six different species of mammal resulted in specific immune responses to both flowlpox antigens and to rabies glycoprotein. In mice, cats and dogs the immune response was sufficient to protect against a live rabies virus challenge. The results demosntrate the utility of a fowlpox virus vector in immunizing non-avian species against rabies in the absence of productive viral replication of the fowlpox vector.