VIRAL DETERMINANTS OF VIRULENCE FOR RIFT-VALLEY FEVER (RVF) IN RATS
VIRAL DETERMINANTS OF VIRULENCE FOR RIFT-VALLEY FEVER (RVF) IN RATS
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DOI：
10.1016/0882-4010(88)90096-4
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发表时间：
1988-10-01
影响因子：
3.8
通讯作者：
PETERS, CJ
中科院分区：
文献类型：
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作者：
ANDERSON, GW;PETERS, CJ
裂谷热病毒株或变种 (RVFV) 的比较 (a) 对 Wistar-Furth 大鼠的毒力； (b) 对大鼠和人干扰素的体外敏感性； (c) 在遗传抗性或易感大鼠品系的原代肝细胞培养物中形成斑块的能力，以及 (d) 在连续大鼠细胞系中的复制潜力。埃及菌株对 Wistar-Furth 大鼠具有高毒力；对大鼠干扰素-α/β相对耐药；能够在原代肝细胞单层中产生斑块；一般来说，比撒哈拉以南低毒株的复制速度更快。事实上，来自撒哈拉以南非洲的所有菌株都对大鼠干扰素敏感，并且不会在大鼠单层肝细胞中形成斑块。津巴布韦的 2269/74 菌株是一个例外，它具有埃及菌株的特征，包括对 Wistar-Furth 大鼠的毒力增强。当在 A-549 细胞上测试人重组干扰素-α 时，RVFV 毒株对大鼠的相对毒力与干扰素敏感性不相关。因此，RVFV 毒株的一些体外表型特征往往与 Wistar-Furth 大鼠的毒力以及病毒毒株的地理起源相关。
Rift Valley fever viral strains or variants (RVFV) were compared with respect to (a) virulence for Wistar-Furth rats; (b) in vitro sensitivity to rat and human interferon; (c) ability to form plaques in primary hepatocyte cultures from genetically resistant or susceptible rat strains, and (d) replicative potential in continuous rat cell lines. Egyptian strains were highly virulent for Wistar-Furth rats; relatively resistant to rat interferon-alpha/beta; capable of producing plaques in primary hepatocyte monolayers; and, in general, replicated more rapidly than the low-virulent, sub-Saharan strains. Virtually all strains from sub-Saharan Africa were sensitive to rat interferon and did not form plaques in rat hepatocyte monolayers. An exception was the 2269/74 strain from Zimbabwe, which had characteristics of the Egyptian strains including increased virulence for Wistar-Furth rats. The relative virulence of RVFV strains for rats did not correlate with interferon sensitivity when human recombinant interferon-alpha was tested on A-549 cells. Thus, several in vitro phenotypic characteristics of RVFV strains tend to correlate with virulence for Wistar-Furth rats and with geographical origin of the viral strains.