A possible disk to cylinder transition of assemblies of feast/famine regulatory proteins, FFRPs.
A possible disk to cylinder transition of assemblies of feast/famine regulatory proteins, FFRPs.
复制标题
盛宴/饥荒调节蛋白 FFRP 组装体可能从圆盘到圆柱体的转变。
DOI：
10.2183/pjab.79b.63
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Masashi Suzuki
中科院分区：
文献类型：
--
作者：
H. Koike;M. Sakuma;Ayaka Mikami;N. Amano;Masashi Suzuki
我们从古细菌火球菌属中结晶了蛋白质 pot0434017。 OT3。在晶体中，该蛋白质的二聚体排列成螺旋柱体，通过交叉不对称单元继续穿过晶体；每个螺旋圈由 6 组二聚体组成。同时，据报道，相似的蛋白质通过 4 组二聚体在晶体中形成圆盘（来自 P. OT3 的 pot1216151 和来自激烈火球菌的 LrpA）。这些古菌蛋白和大肠杆菌转录因子Lrp（亮氨酸负责调节蛋白）和AsnC（天冬酰胺合成C基因产物），与大量其他古菌和真细菌蛋白一起，形成了一个庞大的蛋白家族；为此提出了盛宴/饥荒调节蛋白（FFRP）的分类。在分析 FFRP 组装形式的基础上，并在鉴定各种细菌基因组中编码的 ffrp 基因的数量和类型的基础上，讨论了 FFRP 可能的生物学作用。（由 Masanori OTSUKA, M. J. A. 通讯，2003 年 2 月 12 日）
We have crystallized the protein pot0434017 from the archaeon Pyrococcus sp. OT3. In the crystals dimers of this protein are arranged into helical cylinders, continuing through the crystals by crossing over asymmetric units; 6 sets of dimers forming each helical turn. While, formation of disks in crystals by 4 sets of dimers was reported for similar proteins (pot1216151 from P. OT3 and LrpA from Pyrococcus furiosus). These archaeal proteins and E. coli transcription factors, Lrp (leucine-responsible regulatory protein) and AsnC (aspargine synthesis C gene product), together with a large number of other archaeal and eubacterial proteins, form a large protein family; for which the classification feast/famine regulatory proteins (FFRPs) is proposed. On the basis of an analysis of the assembly forms of FFRPs, and on the basis of identification of numbers and types of ffrp genes coded in various bacterial genomes, possible biological roles of FFRPs are discussed.(Communicated by Masanori OTSUKA, M. J. A., Feb. 12, 2003)