Osteoblast-like cell proliferation on tape-cast and sintered tricalcium phosphate sheets
Osteoblast-like cell proliferation on tape-cast and sintered tricalcium phosphate sheets
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DOI：
10.1016/j.actbio.2007.10.001
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发表时间：
2008-03-01
影响因子：
9.7
通讯作者：
Nishiyama, Norihiro
中科院分区：
文献类型：
--
作者：
Tanimoto, Yasuhiro;Shibata, Yo;Nishiyama, Norihiro
研究了烧结温度对成骨细胞在流延法制备的磷酸三钙(TcP)片材上体外增殖的影响。在900、1000、1100、1150和1200℃五种不同的烧结温度下，在常压下将流延成型的β-磷酸三钙生片在炉中烧结2 h。细胞培养1周后，计数沉积在成骨细胞样细胞(MC3T3-E1)上的细胞数量，发现随着烧结温度的升高，MC3T3-E1细胞的数量增加。在衰减全反射红外光谱中，随着烧结温度的升高，900-1150 cm(-1)处的峰值逐渐减小，并向低波数方向移动。同时，在900℃下烧结的TcP的Zeta电位与其他组相比显示出高度的负电荷。这表明，在900℃下烧结的TCP片材的溶解度越高，从Zeta电位测量获得的负电荷就越高。在本研究范围内，成骨细胞样细胞的增殖随着烧结温度的升高而增加。烧结型聚碳酸酯片材表面的生物稳定性被认为影响了细胞的增殖。(C)2007年Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
The influence of sintering temperature on the in vitro proliferation of osteoblast-like cells to sintered tricalcium phosphate (TCP) sheets prepared by tape-casting was investigated. Green sheets of tape-cast beta-TCP were sintered for 2 h in a furnace at atmospheric pressure at five different sintering temperatures: 900, 1000, 1100, 1150 and 1200 degrees C. The number of ostcoblast-like (MC3T3-E1) cells deposited onto TCP sheets was counted after cell cultivation for I week and was found to have increased with increasing sintering temperature. The TCP sheets sintered at 900 degrees C exhibited a significantly lower cell number than TCP sheets sintered at 1000, 1100, 1150 and 1200 degrees C. In the attenuated total reflection infrared spectra, the peaks around 900-1150 cm(-1), corresponding to the P-O vibration mode of the phosphate group, gradually decreased and shifted to lower wavenumbers with increasing sintering temperature. Meanwhile, the zeta potential of TCP sintered at 900 degrees C showed a highly negative charge when compared with the other groups. This would suggest that the higher solubility of the TCP sheets sintered at 900 degrees C exerted the higher negative charge obtained from zeta potential measurement. Within the limitations of this study, it was indicated that osteoblast-like cell proliferation increased with increasing sintering temperature. The biological stability of the sintered TCP sheet surface was considered to have affected cell proliferation. (C) 2007 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.