Modification of hydrophobic acrylic intraocular lens with poly(ethylene glycol) by atmospheric pressure glow discharge: A facile approach
Modification of hydrophobic acrylic intraocular lens with poly(ethylene glycol) by atmospheric pressure glow discharge: A facile approach
复制标题
通过大气压辉光放电用聚乙二醇改性疏水性丙烯酸人工晶状体：一种简便的方法
DOI：
10.1016/j.apsusc.2010.05.068
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发表时间：
2010-10-01
影响因子：
6.7
通讯作者：
Yao, Ke
中科院分区：
文献类型：
--
作者：
Lin, Lin;Wang, Yao;Yao, Ke
为了方便、连续地改善疏水性丙烯酸人工透镜（IOL）前表面的生物相容性，以氩气为放电气体，采用常压辉光放电（APGD）法对聚乙二醇（PEG）进行固定化。通过静态水接触角和X射线光电子能谱表征IOL表面的亲水性和化学变化，以确认PEG的共价结合。用场发射扫描电子显微镜和原子力显微镜观察人工晶状体表面的形态。通过体外血小板、巨噬细胞和透镜上皮细胞（LECs）粘附实验评价表面生物相容性。结果显示，PEG接枝的IOL的前表面显示出显著且永久改善的亲水性。观察到细胞排斥性，特别是在PEG修饰的IOL组中，其抵抗血小板、巨噬细胞和LEC的附着。此外，细胞的铺展和生长受到抑制，这可能归因于修饰的PEG的空间稳定力和链运动效应。所有这些结果表明，疏水性丙烯酸酯IOL可以通过PEG以方便和连续的方式通过APGD处理进行亲水改性，这将为进一步的工业应用提供优势。(C)2010 Elsevier B.V.保留所有权利。
To improve the anterior surface biocompatibility of hydrophobic acrylic intraocular lens (IOL) in a convenient and continuous way, poly(ethylene glycol)s (PEGs) were immobilized by atmospheric pressure glow discharge (APGD) treatment using argon as the discharge gas. The hydrophilicity and chemical changes on the IOL surface were characterized by static water contact angle and X-ray photoelectron spectroscopy to confirm the covalent binding of PEG. The morphology of the IOL surface was observed under field emission scanning electron microscopy and atomic force microscopy. The surface biocompatibility was evaluated by adhesion experiments with platelets, macrophages, and lens epithelial cells (LECs) in vitro. The results revealed that the anterior surface of the PEG-grafted IOL displayed significantly and permanently improved hydrophilicity. Cell repellency was observed, especially in the PEG-modified IOL group, which resisted the attachment of platelets, macrophages and LECs. Moreover, the spread and growth of cells were suppressed, which may be attributed to the steric stabilization force and chain mobility effect of the modified PEG. All of these results indicated that hydrophobic acrylic IOLs can be hydrophilic modified by PEG through APGD treatment in a convenient and continuous manner which will provide advantages for further industrial applications. (C) 2010 Elsevier B.V. All rights reserved.