Expression of hypoxia-induced semaphorin 7A correlates with the severity of inflammation and osteoclastogenesis in experimentally induced periapical lesions
Expression of hypoxia-induced semaphorin 7A correlates with the severity of inflammation and osteoclastogenesis in experimentally induced periapical lesions
复制标题
缺氧诱导的信号蛋白 7A 的表达与实验诱导的根尖周病变中炎症和破骨细胞生成的严重程度相关。
DOI：
10.1016/j.archoralbio.2016.10.032
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发表时间：
2017-03-01
影响因子：
3
通讯作者：
Bian, Zhuan
中科院分区：
文献类型：
--
作者：
He, Miao;Bian, Zhuan
目的：虽然缺氧和炎症密切相关，但炎症性缺氧对根尖周病变发病机制的影响仍不清楚。本研究的目的是检查实验诱导的大鼠根尖周病变进展过程中的缺氧情况，并得出缺氧诱导的信号蛋白 7A (Sema7a) 表达、炎症严重程度和病变中破骨细胞生成之间的相关性。 设计：40 只 Sprague-Dawley 大鼠下颌第一磨牙牙髓暴露后出现根尖周病变。将动物随机分为四组并在牙髓暴露后0、7、14和28天处死。获取包含第一磨牙的双侧下颌骨并常规制备用于组织学、免疫组织化学、酶组织化学分析和定量聚合酶链反应检测Sema7a mRNA表达。通过单因素方差分析、Pearson相关性和线性趋势检验对数据进行分析。结果：在实验诱导的根尖周病变发展过程中，根尖周组织变得缺氧，HIF-1α阳性细胞数量稳步增加，与病变中Sema7a mRNA的表达呈正相关。此外，Sema7a的表达与炎症浸润程度和破骨细胞数量分别呈显着正相关。结论：缺氧诱导的Sema7a参与了根尖周病变的发病机制，为今后治疗该炎症性疾病提供了新的治疗靶点。 (C) 2016 Elsevier Ltd. 保留所有权利。
Objective: While hypoxia and inflammation are intimately linked, the effects of inflammatory hypoxia on the pathogenesis of periapical lesions remain largely unknown. The aim of this study was to examine hypoxia during the progression of experimentally induced rat periapical lesions, and to derive correlations between hypoxia-induced Semaphorin 7A (Sema7a) expression, severity of inflammation, and osteoclastogenesis in the lesions.Design: Periapical lesions were developed after mandibular first molar pulp exposure in forty Sprague-Dawley rats. The animals were randomly divided into four groups and sacrificed at 0, 7, 14, and 28 days after pulpal exposure. The bilateral mandibles containing the first molar were obtained and routinely prepared for histological, immunohistochemical, enzyme histochemical analyses and quantitative polymerase chain reaction detecting Sema7a mRNA expression. Data were analysed by one-way analysis of variance and the Pearson's correlation and linear tendency test.Results: Periapical tissues become hypoxic during the development of experimentally induced periapical lesions, with steadily increasing numbers of HIF-l alpha-positive cells that positively correlate with the expression of Sema7a mRNA in the lesions. Furthermore, significant positive correlates were derived for the expression of Sema7a and the degree of inflammatory infiltration and osteoclast number, respectively.Conclusions: Hypoxia-induced Sema7a participates in the pathogenesis of periapical lesions, providing a novel therapeutic target for the treatment of this inflammatory disease in the future. (C) 2016 Elsevier Ltd. All rights reserved.