KNDy neurone activation prior to the LH surge of the ewe is disrupted by LPS.
KNDy neurone activation prior to the LH surge of the ewe is disrupted by LPS.
复制标题
母羊 LH 激增之前的 KNDy 神经元激活被 LPS 破坏。
作者：
C. Fergani;J. Routly;D. Jones;L. Pickavance;R. Smith;H. Dobson
在母羊中，类固醇激素作用于下丘脑弓状核（ARC）以启动GnRH/LH峰。在ARC内，类固醇信号转导可由雌激素接受性多巴胺、β-内啡肽或神经肽Y（NPY）表达细胞以及共定位kisspeptin、神经激肽B（NKB）和强啡肽（称为KNDy）的细胞介导。我们研究了卵泡期这些细胞被激活的时间（即，共定位c-Fos）相对于LH峰发作的时间，因此可能参与峰产生机制。此外，我们的目的是阐明这些激活模式是否改变后脂多糖（LPS）管理，这是已知的抑制LH峰。母羊的卵泡期通过黄体酮停药同步，每2小时采集一次血液样本。在有或没有给予LPS（100 ng/kg）的情况下，在卵泡期的不同时间取回下丘脑组织。在性行为开始前，激活的多巴胺细胞的百分比降低，而在LH峰期间，β-内啡肽的激活降低，NPY的激活趋于增加。这些模式不受LPS给药的干扰。最大的共同表达的c-Fos强啡肽免疫反应神经元中观察到早在滤泡期，相比kisspeptin和NKB，这是最大限度地激活在浪涌。这表明ARC强啡肽在LH峰产生机制中的独特作用。急性LPS使强啡肽和kisspeptin免疫反应阳性细胞的百分比降低。因此，在卵巢完整的母羊中，KNDy神经元在LH峰发作之前被激活，并且这种模式被LPS的施用抑制。
In the ewe, steroid hormones act on the hypothalamic arcuate nucleus (ARC) to initiate the GnRH/LH surge. Within the ARC, steroid signal transduction may be mediated by estrogen receptive dopamine-, β-endorphin- or neuropeptide Y (NPY)-expressing cells, as well as those co-localising kisspeptin, neurokinin B (NKB) and dynorphin (termed KNDy). We investigated the time during the follicular phase when these cells become activated (i.e., co-localise c-Fos) relative to the timing of the LH surge onset and may therefore be involved in the surge generating mechanism. Furthermore, we aimed to elucidate whether these activation patterns are altered after lipopolysaccharide (LPS) administration, which is known to inhibit the LH surge. Follicular phases of ewes were synchronised by progesterone withdrawal and blood samples were collected every 2 h. Hypothalamic tissue was retrieved at various times during the follicular phase with or without the administration of LPS (100 ng/kg). The percentage of activated dopamine cells decreased before the onset of sexual behaviour, whereas activation of β-endorphin decreased and NPY activation tended to increase during the LH surge. These patterns were not disturbed by LPS administration. Maximal co-expression of c-Fos in dynorphin immunoreactive neurons was observed earlier during the follicular phase, compared to kisspeptin and NKB, which were maximally activated during the surge. This indicates a distinct role for ARC dynorphin in the LH surge generation mechanism. Acute LPS decreased the percentage of activated dynorphin and kisspeptin immunoreactive cells. Thus, in the ovary-intact ewe, KNDy neurones are activated prior to the LH surge onset and this pattern is inhibited by the administration of LPS.
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绵羊大脑中雌激素受体免疫反应细胞的分布。
影响因子：
4.8
作者：
Lehman,MN;Ebling,FJ;Moenter,SM;Karsch,FJ
通讯作者：
Karsch,FJ
影响因子：
4.8
作者：
J. Z. Kasa-Vubu;G E Dahl;N. P. Evans;L. Thrun;S. Moenter;Vasantha Padmanabhan;F. Karsch
通讯作者：
J. Z. Kasa-Vubu;G E Dahl;N. P. Evans;L. Thrun;S. Moenter;Vasantha Padmanabhan;F. Karsch
DOI：
10.1152/ajpendo.00517.2010
发表时间：
2011-01-01
影响因子：
5.1
作者：
Navarro, Victor M.;Castellano, Juan M.;Steiner, Robert A.
通讯作者：
Steiner, Robert A.
DOI：
10.1073/pnas.88.23.10931
发表时间：
1991-12-01
影响因子：
11.1
作者：
KALRA, SP;DUBE, MG;KALRA, PS
通讯作者：
KALRA, PS
影响因子：
3.6
作者：
KARSCH, FJ;CUMMINS, JT;CLARKE, IJ
通讯作者：
CLARKE, IJ