CYTO-TOXICITY, MUTAGENICITY AND DNA DAMAGE BY HOECHST-33342
CYTO-TOXICITY, MUTAGENICITY AND DNA DAMAGE BY HOECHST-33342
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DOI：
10.1177/30.2.7061816
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发表时间：
1982-01-01
影响因子：
3.2
通讯作者：
OLIVE, PL
中科院分区：
文献类型：
--
作者：
DURAND, RE;OLIVE, PL
Hoechst 33342可用于活的中国仓鼠细胞的DNA含量的流动显微荧光测定法（FMF）分析，给出良好的分辨率（变异系数（CV））。6%）。然而，染料是一种非常有效的DNA合成抑制剂，在比最佳FMF分辨率所需的浓度低10倍的浓度下观察到V79细胞的DNA合成速率显著降低。无毒和最低毒性Hoechst浓度也导致可证实的突变，如通过6-硫鸟嘌呤抗性测定的。Hoechst消失从细胞返回到正常的生长培养基染色后表明2个组件的结合，因为约一半的总染色迅速删除，而其余的显然是稀释的细胞分裂。当保持在4 ℃时，含有Hoechst 33342的细胞比对照细胞死亡更快。C，并且如果在. gtorsim下操作，也更容易被UV激光束灭活。100 mV电源。因此，Hoechst 33342可以提供关于活细胞的DNA含量的信息，但以最小的毒性、中等的突变和显著的细胞周期扰动为代价。
Hoechst 33342 can be used for flow microfluorimetry (FMF) analysis of the DNA content of living Chinese hamster cells, giving good resolution (coefficients of variation (CVs) .apprx. 6%) with relatively nontoxic staining regimens. The dye is, however, a very efficient inhibitor of DNA synthesis, with a marked depression of the DNA synthetic rate for V79 cells observed at concentrations 10-fold less than those required for optimal FMF resolution. Nontoxic and minimally toxic Hoechst concentrations also resulted in demonstrable mutation, as assayed by 6-thioguanine resistance. Hoechst disappearance from cells returned to normal growth medium after staining suggests 2 components of binding, since about half of the total stain is rapidly removed, whereas the rest is apparently diluted only by cell division. Cells containing Hoechst 33342 die more rapidly than control cells when held at 4.degree. C and are also more susceptible to inactivation by the UV laser beam if operated at .gtorsim. 100 mV power. Thus, Hoechst 33342 can provide information about the DNA content of living cells, but at the expense of minimal toxicity, moderate mutation and significant cell cycle perturbations.